Download MendeleyCofolding organizes alfalfa mosaic virus RNA and coat protein for replication.
Guogas, L.M., Filman, D.J., Hogle, J.M., Gehrke, L.(2004) Science 306: 2108-2111
- PubMed: 15604410
- DOI: https://doi.org/10.1126/science.1103399
- Primary Citation of Related Structures:
1XOK - PubMed Abstract:
Alfalfa mosaic virus genomic RNAs are infectious only when the viral coat protein binds to the RNA 3' termini. The crystal structure of an alfalfa mosaic virus RNA-peptide complex reveals that conserved AUGC repeats and Pro-Thr-x-Arg-Ser-x-x-Tyr coat protein amino acids cofold upon interacting. Alternating AUGC residues have opposite orientation, and they base pair in different adjacent duplexes. Localized RNA backbone reversals stabilized by arginine-guanine interactions place the adenosines and guanines in reverse order in the duplex. The results suggest that a uniform, organized 3' conformation, similar to that found on viral RNAs with transfer RNA-like ends, may be essential for replication.
Organizational Affiliation:
Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, MA 02115, USA.
