T7 DNA Polymerase Complexed To DNA Primer/Template and ddATP     DOI:10.2210/pdb1skr/pdb   NDB ID: PD0522

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Primary Citation       Molecular Description Hide Classification: Transferase/electron Transport/dna Structure Weight: 105987.68   Molecule: 5'-D(*CP*GP*AP*AP*AP*AP*CP*GP*AP*C*GP*GP*CP*CP*AP*GP*TP*GP*CP*CP*AP*(2DA))-3' Polymer: 1 Type: polydeoxyribonucleotide Length: 22 Chains: P Other Details: DNA primer Molecule: 5'-D(*CP*CP*CP*TP*TP*TP*GP*GP*CP*AP*CP*TP*GP*GP*CP*CP*GP*TP*CP*GP*TP*TP*TP*TP*CP*G)-3' Polymer: 2 Type: polydeoxyribonucleotide Length: 26 Chains: T Other Details: DNA template Molecule: DNA polymerase Polymer: 3 Type: polypeptide(L) Length: 698 Chains: A EC#: 2.7.7.7    Mutation: DEL(118-123) Molecule: Thioredoxin 1 Polymer: 4 Type: polypeptide(L) Length: 108 Chains: B       Source Hide Polymer: 1 Scientific Name: Synthetic construct   Polymer: 2 Scientific Name: Synthetic construct   Polymer: 3 Scientific Name: Enterobacteria phage t7 Expression System: Escherichia coli   Polymer: 4 Scientific Name: Escherichia coli Expression System: Escherichia coli         Related PDB Entries Hide Id Details 1SKS  Binary 3' complex of T7 DNA polymerase with a DNA primer/template containing a cis-syn thymine dimer on the template  1SKW  Binary 3' complex of T7 DNA polymerase with a DNA primer/template containing a disordered cis-syn thymine dimer on the template  1SL0  Ternary 3' complex of T7 DNA polymerase with a DNA primer/template containing a disordered cis-syn thymine dimer on the template and an incoming nucleotide  1SL1  Binary 5' complex of T7 DNA polymerase with a DNA primer/template containing a cis-syn thymine dimer on the template  1SL2  Ternary 5' complex of T7 DNA polymerase with a DNA primer/template containing a cis-syn thymine dimer on the template and an incoming nucleotide  1T7P  T7 DNA POLYMERASE COMPLEXED TO DNA PRIMER/TEMPLATE, A NUCLEOSIDE TRIPHOSPHATE, AND ITS PROCESSIVITY FACTOR THIOREDOXIN        Ligand Chemical Component Hide Identifier Name Formula Interaction View Links DAD     2',3'-DIDEOXYADENOSINE-5'-TRIPHOSPHATE C10 H16 N5 O11 P3 Ligand Explorer MG     MAGNESIUM ION Mg Ligand Explorer       Modified Residues Hide Identifier Name Formula Interaction View Links 2DA 2',3'-DIDEOXYADENOSINE-5'-MONOPHOSPHATE (parent: DA)(type: dnaLinking) C10 H14 N5 O5 P Ligand Explorer       Derived Data Hide SCOP Classification v1.75 - (3 Domains) CATH Classification v3.2.0 - (5 Domains) PFAM Classification - (2 Domains) GO Terms - (17 Terms)     Reset Layout

Biological Assembly     More Images... View in Jmol Other Viewers close KiNG WebMol QuickPDB FirstGlance Kiosk SimpleViewer Protein Workshop Ligand Explorer Biological assembly assigned by authors         Deposition Summary Hide Authors:   Li, Y.,   Dutta, S.,   Doublie, S.,   Bdour, H.M.,   Taylor, J.S.,   Ellenberger, T. Deposition:   2004-03-05 Release:   2004-07-06 Last Modified (REVDAT):   2009-02-24       Experimental Details Hide Method:   X-RAY DIFFRACTION Experimental Data:    [ EDS ] Resolution[Å]: 2.40 R-Value: 0.226 (work) R-Free: 0.270 Space Group: P 21 21 2 Unit Cell:   Length [Å] Angles [°] a = 105.51 α = 90.00  b = 214.02 β = 90.00  c = 52.04 γ = 90.00        Structure Links Hide Molecule of the Month Features: Thymine Dimers