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Crystal structure of restriction endonuclease BGLII complexed with DNA 16-mer
DOI:10.2210/pdb1dfm/pdb   NDB ID: PD0101
1DFM
Primary Citation
 
 
  •   Molecular Description Hide
    Classification: Hydrolase/dna
    Structure Weight: 61934.70
    Molecule: DNA (5'-D(*TP*AP*TP*TP*AP*TP*AP*GP*AP*TP*CP*TP*AP*TP*AP*A)-3')
    Polymer: 1 Type: dna Length: 16
    Chains: C, D
    Molecule: ENDONUCLEASE BGLII
    Polymer: 2 Type: protein Length: 223
    Chains: A, B
    EC#: 3.1.21.4   
    Fragment: BGLII
    Mutation: SELENOMETHIONYL (MSE FOR MET)
    Organism Bacillus subtilis
    Gene Name bglIIR
    UniProtKB:   Protein Feature View | Search PDB | Q45488  
     
  •   Structure Validation Hide

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  •   Related Citations in PDB Entry (REMARK 1) Hide
     
  •   Source Hide
    Polymer: 1
    Scientific Name: Synthetic construct   Taxonomy    
    Polymer: 2
    Scientific Name: Bacillus subtilis   Taxonomy   Expression System: Escherichia coli  
     
  •   Related PDB Entries Hide
    Identifier Details
    1BHM  BAMHI + DNA 
    1D2I  BGLII + DNA AT 1.7 ANGSTROMS 
     
  •   Ligand Chemical Component Hide
    Identifier Formula Name View Interactions
    CA
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    CA Ca
    CALCIUM ION
     
  •   Modified Residues Hide
    Identifier Formula Parent Type
    MSE
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    MSE C5 H11 N O2 Se MET lPeptideLinking
     
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  • Nucleic Acid Database Hide
    View the NDB ID associated with this structure: PD0101
     
 
Data in orange boxes are gathered from external resources (when available).
 
  Biological Assembly       
Biological assembly 1 assigned by authors
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  •   Deposition Summary Hide
    Authors:   Lukacs, C.M.,  Kucera, R.,  Schildkraut, I.,  Aggarwal, A.K.

    Deposition:   1999-12-06
    Release:   2000-02-21
    Last Modified (REVDAT):   2009-02-24
     
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    2011-07-13
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  •   Experimental Details Hide
    Method:   X-RAY DIFFRACTION
    Exp. Data:
    N/A
    Resolution[Å]:   1.50
    R-Value: 0.192 (obs.)
    R-Free: 0.224
    Space Group: P 21 21 21
    Unit Cell:
      Length [Å] Angles [°]
    a = 48.70 α = 90.00 
    b = 101.80 β = 90.00 
    c = 116.90 γ = 90.00