1AOI

COMPLEX BETWEEN NUCLEOSOME CORE PARTICLE (H3,H4,H2A,H2B) AND 146 BP LONG DNA FRAGMENT


Experimental Data Snapshot

  • Method: X-RAY DIFFRACTION
  • Resolution: 2.80 Å
  • R-Value Free: 0.302 
  • R-Value Work: 0.224 

wwPDB Validation   3D Report Full Report


This is version 1.3 of the entry. See complete history


Literature

Crystal structure of the nucleosome core particle at 2.8 A resolution.

Luger, K.Mader, A.W.Richmond, R.K.Sargent, D.F.Richmond, T.J.

(1997) Nature 389: 251-260

  • DOI: https://doi.org/10.1038/38444
  • Primary Citation of Related Structures:  
    1AOI

  • PubMed Abstract: 

    The X-ray crystal structure of the nucleosome core particle of chromatin shows in atomic detail how the histone protein octamer is assembled and how 146 base pairs of DNA are organized into a superhelix around it. Both histone/histone and histone/DNA interactions depend on the histone fold domains and additional, well ordered structure elements extending from this motif. Histone amino-terminal tails pass over and between the gyres of the DNA superhelix to contact neighbouring particles. The lack of uniformity between multiple histone/DNA-binding sites causes the DNA to deviate from ideal superhelix geometry.


  • Organizational Affiliation

    Institut für Molekularbiologie und Biophysik, Zürich, Switzerland.


Macromolecules

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Entity ID: 2
MoleculeChains Sequence LengthOrganismDetailsImage
HISTONE H3C [auth A],
G [auth E]
116Xenopus laevisMutation(s): 0 
UniProt
Find proteins for P02302 (Xenopus laevis)
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UniProt GroupP02302
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Entity ID: 3
MoleculeChains Sequence LengthOrganismDetailsImage
HISTONE H4D [auth B],
H [auth F]
87Xenopus laevisMutation(s): 0 
UniProt
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Entity ID: 4
MoleculeChains Sequence LengthOrganismDetailsImage
HISTONE H2AE [auth C],
I [auth G]
116Xenopus laevisMutation(s): 0 
UniProt
Find proteins for P06897 (Xenopus laevis)
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Entity ID: 5
MoleculeChains Sequence LengthOrganismDetailsImage
HISTONE H2BF [auth D],
J [auth H]
99Xenopus laevisMutation(s): 0 
UniProt
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Entity ID: 1
MoleculeChains LengthOrganismImage
PALINDROMIC 146 BP DNA REPEAT 8/9 FROM HUMAN X-CHROMOSOME ALPHA SATELLITE DNAA [auth I],
B [auth J]
146N/A
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  • Reference Sequence
Experimental Data & Validation

Experimental Data

  • Method: X-RAY DIFFRACTION
  • Resolution: 2.80 Å
  • R-Value Free: 0.302 
  • R-Value Work: 0.224 
  • Space Group: P 21 21 21
Unit Cell:
Length ( Å )Angle ( ˚ )
a = 106.04α = 90
b = 181.78β = 90
c = 110.12γ = 90
Software Package:
Software NamePurpose
LOCALmodel building
X-PLORrefinement
IN-HOUSEdata reduction
IN-HOUSEdata scaling
IN-HOUSEphasing

Structure Validation

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Entry History 

Deposition Data

Revision History  (Full details and data files)

  • Version 1.0: 1998-09-30
    Type: Initial release
  • Version 1.1: 2008-05-22
    Changes: Version format compliance
  • Version 1.2: 2011-07-13
    Changes: Version format compliance
  • Version 1.3: 2024-02-07
    Changes: Data collection, Database references, Derived calculations