FibrinNovember 2006 Molecule of the Month by David Goodsell
doi: 10.2210/rcsb_pdb/mom_2006_11 (PDF Version, ePub Version )
When you cut yourself, you bleed, but the bleeding rapidly stops. Blood has a built-in emergency repair system that quickly blocks any damage to the circulatory system, creating a temporary patch that allows time for more permanent repairs. Three basic mechanisms are at work. First, platelets (small fragments of blood cells that circulate in the blood) clump at the site of the wound, forming a weak plug. Second, neighboring blood vessels constrict, reducing the amount of blood flowing into the area. Finally, the protein fibrin assembles into a tough network that clots the blood and forms an insoluble blockage. Together, these methods stop the loss of blood and create a sturdy scab to protect the area as you heal.
Fibrin is normally present in an inactive form known as fibrinogen. Fibrinogen is soluble in water and is found in high concentrations in the blood, where it waits until needed to form a clot. When given the signal, fibrinogen is converted to fibrin, which then assembles into an extended network of fibers. This changes normally-fluid blood into a jelly-like solid, which then dries to form a scab. Of course, it is very important to assemble fibrin networks only in the local area of the cut, and nowhere else, since the blood must continue flowing to other parts of the body. The fine-tuned control of blood clotting is controlled by a cascade of specialized proteins, as discussed in previous installments of the Molecule of the Month on thrombin and tissue factor.
Fibrin is a large, flexible protein composed of six proteins chains. Since it has several flexible parts, it has been difficult to study, and many of the structures in the PDB contain only part of the molecule. Two PDB structures were used to create this picture: 1m1j for the central core of the molecule, and 2baf for the two flexible arms at either side. The four little chains shown with dotted lines at the center, which unfortunately are not seen in the structure analyses, are the key to the activation of fibrin. The ends of these four chains are clipped by thrombin, changing inactive fibrinogen to active fibrin. As shown on the next page, these flexible chains then form the linkages that glue many fibrin chains into a fibril.
Forming the Fibril
Once fibrin is activated, it assembles to form tough fibrils. The chains at the center reach over and bind into pockets on the large globular heads of neighboring fibrin molecules. Also, fibrin associates head-to-head, as shown in the upper structure from PDB entry 1fzc. This head-to-head interaction is made stronger in a clot by crosslinking, making the interaction permanent. Finally, the longer chains colored orange here can extend over to neighboring fibrils to form even larger structures, binding in a small pocket colored green here.
Exploring the Structure
shows fibrin in the process of being activated by thrombin. Only the
center part of fibrin is included in the structure--you have to imagine the rest of the
molecule extended right and left. Thrombin (blue) binds on the two sides of the central
knob in fibrinogen, placing its active site in perfect proximity to the flexible chains that
have to be cleaved. The small green molecules are inhibitors bound in the active site of
This picture was created with RasMol. You can create similar images by clicking on the accession codes here and picking one of the options under Images and Visualization.
Additional Reading on Fibrin
M. W. Mosesson, K. R. Siebenlist and D. A. Meh (2001) The structure and biological
features of fibrinogen and fibrin. Annals of the New York Academy of Sciences 936, 11-
R. F. Doolittle, Z. Yang and I. Mochalkin (2001) Crystal structure studies on fibrinogen and fibrin. Annals of the New York Academy of Sciences 936, 31-43.
M. W. Mosesson (2005) Fibrinogen and fibrin structure and functions. Journal of Thrombosis and Haemostasis 3, 1894-1904.
© 2013 David Goodsell & RCSB Protein Data Bank