Serum AlbuminJanuary 2003 Molecule of the Month by David Goodsell
doi: 10.2210/rcsb_pdb/mom_2003_1 (PDF Version, ePub Version )
Think about how convenient it is to be able to eat. Each one of your ten trillion cells requires a constant supply of nourishment. But we don't have to worry about this--we merely eat our dinner and our body does the rest. The food is digested and the useful pieces are delivered to cells throughout the body, using the bloodstream as the delivery system. Delivery of water-soluble molecules, like sugar, is easy. They float in the watery bloodstream and are picked up by cells along the way. Other important nutrients, however, are not soluble in water, so special carriers must be made to chaperone them to hungry cells.
Carrying Fatty Acids
Serum albumin, shown here from PDB entry 1e7i, is the carrier of fatty acids in the blood. Fatty acids are essential for two major things in your body. They are the building blocks for lipids, which form all of the membranes around and inside cells. They are also rich sources of energy, and may be broken down inside cells to form ATP. Thus, your body maintains a storehouse of fatty acids, stored as fat. When your body needs energy or needs building materials, fat cells release fatty acids into the blood. There, they are picked up by serum albumin and delivered to distant parts of the body.
A Versatile Protein
Serum albumin is the most plentiful protein in blood plasma. Each protein molecule can carry seven fatty acid molecules. They bind in deep crevices in the protein, burying their carbon-rich tails safely away from the surrounding water. Serum albumin also binds to many other water-insoluble molecules. In particular, serum albumin binds to many drug molecules, such as ibuprofin, and can strongly affect the way they are delivered through the body.
A Generic Protein
Since serum albumin is so common in the blood and so easy to purify, it was one of the first proteins to be studied by scientists. Today, the similar protein from cows--bovine serum albumin or BSA--is widely used in research when a generic protein is needed. Many enzymes are unstable when they are placed in a dilute solution, so it is difficult to study them in the laboratory. One solution is to add some BSA. It stabilizes the enzyme during the experiment, but it is relatively neutral so it doesn't affect the properties of the enzyme.
A Collection of Carriers
Many different molecules are transported in the blood, so it is no surprise that we make a diverse collection of proteins to carry them. Unlike serum albumin, many of these are specific carriers, delivering only a single type of molecule. Two examples are shown here. Transferrin, shown here from PDB entry 1h76, carries iron ions and transthyretin, from PDB entry 1tha, delivers thyroid hormones. The blood is filled with these busy carriers that deliver their cargo throughout the body.
Exploring the Structure
A series of human serum albumin structures that show how different fatty acids bind to the protein are available in the PDB. On the first page of this Molecule of the Month, there are seven molecules of a saturated fatty acid bound to the protein. Some can be seen peeking out from the surface. The structure shown here, from PDB entry 1gnj, has seven molecules of arachidonic acid bound to it. The protein is shown with blue tubes and the fatty acids are shown with spheres at each atom. As you explore this structure, notice how the protein chain surrounds the carbon-rich tails of the fatty acids, shielding them from the surrounding water. Arachidonic acid is an unsaturated fatty acid with several double bonds that form rigid kinks in the carbon chain. It is important for the construction of molecular messengers used to signal pain and inflammation.
Additional reading about serum albumin
Stephen Curry, Peter Brick and Nicholas P. Franks (1999): Fatty Acid Binding to Human
Serum Albumin: New Insights from Crystallographic Studies. Biochimica et Biophysica Acta 1441, pp. 131-140.
The Albumin Website
Features discoveries regarding serum albumin, an updated list of published albumin mutations with references, and other information of interest related to albumin
© 2013 David Goodsell & RCSB Protein Data Bank