7C7L

Cryo-EM structure of the Cas12f1-sgRNA-target DNA complex


ELECTRON MICROSCOPY

Refinement

RMS Deviations
KeyRefinement Restraint Deviation
r_long_range_B_refined37.932
r_long_range_B_other37.932
r_dihedral_angle_2_deg37.337
r_dihedral_angle_3_deg20.774
r_dihedral_angle_4_deg18.117
r_scangle_other17.732
r_mcangle_other14.234
r_mcangle_it14.233
r_scbond_it10.516
r_scbond_other10.516
RMS Deviations
KeyRefinement Restraint Deviation
r_long_range_B_refined37.932
r_long_range_B_other37.932
r_dihedral_angle_2_deg37.337
r_dihedral_angle_3_deg20.774
r_dihedral_angle_4_deg18.117
r_scangle_other17.732
r_mcangle_other14.234
r_mcangle_it14.233
r_scbond_it10.516
r_scbond_other10.516
r_mcbond_it8.891
r_mcbond_other8.881
r_dihedral_angle_1_deg7.596
r_angle_refined_deg1.498
r_angle_other_deg1.385
r_chiral_restr0.076
r_bond_refined_d0.007
r_gen_planes_refined0.007
r_gen_planes_other0.002
r_bond_other_d
r_nbd_refined
r_nbd_other
r_nbtor_refined
r_nbtor_other
r_xyhbond_nbd_refined
r_xyhbond_nbd_other
r_metal_ion_refined
r_metal_ion_other
r_symmetry_vdw_refined
r_symmetry_vdw_other
r_symmetry_hbond_refined
r_symmetry_hbond_other
r_symmetry_metal_ion_refined
r_symmetry_metal_ion_other
r_scangle_it
r_rigid_bond_restr
r_sphericity_free
r_sphericity_bonded
Sample
Cryo-EM structure of the Cas12f1-sgRNA-target DNA complex
Sample Components
Cas12f1
sgRNA
target DNA
Specimen Preparation
Sample Aggregation StatePARTICLE
Vitrification Instrument
Cryogen NameETHANE
Sample Vitrification Details
3D Reconstruction
Reconstruction MethodSINGLE PARTICLE
Number of Particles87253
Reported Resolution (Å)3.3
Resolution MethodFSC 0.143 CUT-OFF
Other Details
Refinement Type
Symmetry TypePOINT
Point SymmetryC1
Map-Model Fitting and Refinement
Id1
Refinement Space
Refinement Protocol
Refinement Target
Overall B Value
Fitting Procedure
Details
Data Acquisition
Detector TypeGATAN K3 (6k x 4k)
Electron Dose (electrons/Å**2)48.7
Imaging Experiment1
Date of Experiment
Temperature (Kelvin)
Microscope ModelFEI TITAN KRIOS
Minimum Defocus (nm)
Maximum Defocus (nm)
Minimum Tilt Angle (degrees)
Maximum Tilt Angle (degrees)
Nominal CS
Imaging ModeBRIGHT FIELD
Specimen Holder Model
Nominal Magnification
Calibrated Magnification
SourceFIELD EMISSION GUN
Acceleration Voltage (kV)300
Imaging Details
EM Software
TaskSoftware PackageVersion
IMAGE ACQUISITIONSerialEM
CTF CORRECTIONRELION3.0
CTF CORRECTIONCTFFIND4.1.13
INITIAL EULER ASSIGNMENTRELION3.0
FINAL EULER ASSIGNMENTRELION3.0
Image Processing
CTF Correction TypeCTF Correction DetailsNumber of Particles SelectedParticle Selection Details
PHASE FLIPPING AND AMPLITUDE CORRECTION