NMR Experiment |
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Experiment | Type | Sample Contents | Solvent | Ionic Strength | pH | Pressure | Temperature (K) | Spectrometer |
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1 | 2D 1H-15N TROSY-HSQC | 0.7 mM [U-13C; U-15N; U-85% 2H] HIV-1 gp41 TMD-CT(LLP2), 40 mM MES, 40 mM DMPC, 80 mM DHPC | 90% H2O/10% D2O | 0 M | 6.7 | 1 atm | 308 | Bruker AVANCE III HD 600 |
2 | 3D TROSY HNCO | 0.7 mM [U-13C; U-15N; U-85% 2H] HIV-1 gp41 TMD-CT(LLP2), 40 mM MES, 40 mM DMPC, 80 mM DHPC | 90% H2O/10% D2O | 0 M | 6.7 | 1 atm | 308 | Bruker AVANCE III HD 600 |
3 | 3D TROSY HNCA | 0.7 mM [U-13C; U-15N; U-85% 2H] HIV-1 gp41 TMD-CT(LLP2), 40 mM MES, 40 mM DMPC, 80 mM DHPC | 90% H2O/10% D2O | 0 M | 6.7 | 1 atm | 308 | Bruker AVANCE III HD 600 |
4 | 2D 1H-15N TROSY-HSQC | 0.7 mM [U-13C; U-15N; U-85% 2H] HIV-1 gp41 TMD-CT(LLP2), 40 mM MES, 40 mM DMPC, 80 mM DHPC | 90% H2O/10% D2O | 0 M | 6.7 | 1 atm | 308 | Bruker AVANCE III 750 |
5 | 3D TROSY HN(CA)CO | 0.7 mM [U-13C; U-15N; U-85% 2H] HIV-1 gp41 TMD-CT(LLP2), 40 mM MES, 40 mM DMPC, 80 mM DHPC | 90% H2O/10% D2O | 0 M | 6.7 | 1 atm | 308 | Bruker AVANCE III 750 |
6 | 3D TROSY HN(CO)CA | 0.7 mM [U-13C; U-15N; U-85% 2H] HIV-1 gp41 TMD-CT(LLP2), 40 mM MES, 40 mM DMPC, 80 mM DHPC | 90% H2O/10% D2O | 0 M | 6.7 | 1 atm | 308 | Bruker AVANCE III 750 |
7 | 3D TROSY HNCACB | 0.7 mM [U-13C; U-15N; U-85% 2H] HIV-1 gp41 TMD-CT(LLP2), 40 mM MES, 40 mM DMPC, 80 mM DHPC | 90% H2O/10% D2O | 0 M | 6.7 | 1 atm | 308 | Bruker AVANCE III 750 |
8 | 2D 1H-15N TROSY-HSQC | 0.8 mM [U-15N; U-2H] HIV-1 gp41 TMD-CT(LLP2), 40 mM MES, 40 mM DMPC, 80 mM DHPC | 90% H2O/10% D2O | 0 M | 6.7 | 1 atm | 308 | Bruker AVANCE III 750 |
9 | 3D 15N-edited NOESY-TROSY-HSQC | 0.8 mM [U-15N; U-2H] HIV-1 gp41 TMD-CT(LLP2), 40 mM MES, 40 mM DMPC, 80 mM DHPC | 90% H2O/10% D2O | 0 M | 6.7 | 1 atm | 308 | Bruker AVANCE III 750 |
10 | 2D 1H-15N TROSY-HSQC | 1.0 mM [U-13C; U-15N] HIV-1 gp41 TMD-CT(LLP2), 40 mM MES, 40 mM DMPC, 80 mM DHPC | 90% H2O/10% D2O | 0 M | 6.7 | 1 atm | 308 | Bruker AVANCE III 800 |
11 | 2D 1H-13C HSQC | 1.0 mM [U-13C; U-15N] HIV-1 gp41 TMD-CT(LLP2), 40 mM MES, 40 mM DMPC, 80 mM DHPC | 90% H2O/10% D2O | 0 M | 6.7 | 1 atm | 308 | Bruker AVANCE III 800 |
12 | 3D 15N-edited NOESY-TROSY-HSQC | 1.0 mM [U-13C; U-15N] HIV-1 gp41 TMD-CT(LLP2), 40 mM MES, 40 mM DMPC, 80 mM DHPC | 90% H2O/10% D2O | 0 M | 6.7 | 1 atm | 308 | Bruker AVANCE III 800 |
13 | 3D 13C-edited NOESY | 1.0 mM [U-13C; U-15N] HIV-1 gp41 TMD-CT(LLP2), 40 mM MES, 40 mM DMPC, 80 mM DHPC | 90% H2O/10% D2O | 0 M | 6.7 | 1 atm | 308 | Bruker AVANCE III 800 |
14 | 2D 1H-15N TROSY-HSQC | 0.5 mM [U-15N; U-2H] HIV-1 gp41 TMD-CT(LLP2), 40 mM MES, 40 mM DMPC, 80 mM DHPC | 90% H2O/10% D2O | 0 M | 6.7 | 1 atm | 308 | Bruker AVANCE III 900 |
15 | 3D 15N-edited NOESY-TROSY-HSQC | 0.5 mM [U-15N; U-2H] HIV-1 gp41 TMD-CT(LLP2), 40 mM MES, 40 mM DMPC, 80 mM DHPC | 90% H2O/10% D2O | 0 M | 6.7 | 1 atm | 308 | Bruker AVANCE III 900 |
16 | 2D 1H-15N TROSY-HSQC | 0.5 mM [U-15N; U-2H] HIV-1 gp41 TMD-CT (LLP2), 0.5 mM [U-13C] HIV-1 gp41 TMD-CT(LLP2), 40 mM MES, 40 mM DMPC, 80 mM DHPC | 90% H2O/10% D2O | 0 M | 6.7 | 1 atm | 308 | Bruker AVANCE III 900 |
17 | 2D 1H-13C HSQC | 0.5 mM [U-15N; U-2H] HIV-1 gp41 TMD-CT (LLP2), 0.5 mM [U-13C] HIV-1 gp41 TMD-CT(LLP2), 40 mM MES, 40 mM DMPC, 80 mM DHPC | 90% H2O/10% D2O | 0 M | 6.7 | 1 atm | 308 | Bruker AVANCE III 900 |
18 | 3D 15N-edited NOESY-TROSY-HSQC | 0.5 mM [U-15N; U-2H] HIV-1 gp41 TMD-CT (LLP2), 0.5 mM [U-13C] HIV-1 gp41 TMD-CT(LLP2), 40 mM MES, 40 mM DMPC, 80 mM DHPC | 90% H2O/10% D2O | 0 M | 6.7 | 1 atm | 308 | Bruker AVANCE III 900 |
19 | 2D 1H-15N TROSY-HSQC | 0.5 mM [U-15N; U-2H] HIV-1 gp41 TMD-CT (LLP2), 0.5 mM [U-13C] HIV-1 gp41 TMD-CT(LLP2), 40 mM MES, 40 mM DMPC, 80 mM DHPC | 90% H2O/10% D2O | 0 M | 6.7 | 1 atm | 308 | Bruker AVANCE III 800 |
20 | 2D 1H-13C HSQC | 0.5 mM [U-15N; U-2H] HIV-1 gp41 TMD-CT (LLP2), 0.5 mM [U-13C] HIV-1 gp41 TMD-CT(LLP2), 40 mM MES, 40 mM DMPC, 80 mM DHPC | 90% H2O/10% D2O | 0 M | 6.7 | 1 atm | 308 | Bruker AVANCE III 800 |
21 | 3D J(CH)-modulated NOESY | 0.5 mM [U-15N; U-2H] HIV-1 gp41 TMD-CT (LLP2), 0.5 mM [U-13C] HIV-1 gp41 TMD-CT(LLP2), 40 mM MES, 40 mM DMPC, 80 mM DHPC | 90% H2O/10% D2O | 0 M | 6.7 | 1 atm | 308 | Bruker AVANCE III 800 |
22 | 2D 1H-15N TROSY-HSQC | 0.5 mM [U-15N; U-85% 2H] HIV-1 gp41 TMD-CT(LLP2), 40 mM MES, 40 mM DMPC, 80 mM DHPC | 90% H2O/10% D2O | 0 M | 6.7 | 1 atm | 308 | Bruker AVANCE III HD 600 |