6L3V

The R15G mutant of human Cx31.3/GJC3 connexin hemichannel


ELECTRON MICROSCOPY

Refinement

RMS Deviations
KeyRefinement Restraint Deviation
f_dihedral_angle_d19.3978
f_angle_d0.8002
f_chiral_restr0.0358
f_plane_restr0.0046
f_bond_d0.004
Sample
The R15G mutant of human Cx31.3/GJC3 connexin hemichannel
Specimen Preparation
Sample Aggregation StatePARTICLE
Vitrification Instrument
Cryogen NameETHANE
Sample Vitrification Details
3D Reconstruction
Reconstruction MethodSINGLE PARTICLE
Number of Particles205646
Reported Resolution (Å)2.63
Resolution MethodFSC 0.143 CUT-OFF
Other Details
Refinement Type
Symmetry TypePOINT
Point SymmetryC6
Map-Model Fitting and Refinement
Id1
Refinement Space
Refinement Protocol
Refinement Target
Overall B Value
Fitting Procedure
Details
Data Acquisition
Detector TypeFEI FALCON III (4k x 4k)
Electron Dose (electrons/Å**2)45
Imaging Experiment1
Date of Experiment
Temperature (Kelvin)
Microscope ModelFEI TITAN KRIOS
Minimum Defocus (nm)
Maximum Defocus (nm)
Minimum Tilt Angle (degrees)
Maximum Tilt Angle (degrees)
Nominal CS
Imaging ModeBRIGHT FIELD
Specimen Holder Model
Nominal Magnification
Calibrated Magnification
SourceFIELD EMISSION GUN
Acceleration Voltage (kV)300
Imaging Details
EM Software
TaskSoftware PackageVersion
PARTICLE SELECTIONcryoSPARC2.0
IMAGE ACQUISITIONEPU1.11.1.50
CTF CORRECTIONGctf1.06
MODEL FITTINGCoot0.8.9.1
MODEL REFINEMENTPHENIX1.14
INITIAL EULER ASSIGNMENTRELION3.0
FINAL EULER ASSIGNMENTcryoSPARC2.0
CLASSIFICATIONcryoSPARC2.0
RECONSTRUCTIONcryoSPARC2.0
Image Processing
CTF Correction TypeCTF Correction DetailsNumber of Particles SelectedParticle Selection Details
PHASE FLIPPING AND AMPLITUDE CORRECTION