NMR Experiment |
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Experiment | Type | Sample Contents | Solvent | Ionic Strength | pH | Pressure | Temperature (K) | Spectrometer |
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1 | 3D HNCA | 0.5 mM [U-13C; U-15N] Tsr2(1-152), 0.5 mM S26A(100-119), 20 mM sodium phosphate, 1 mM [U-2H] DTT, 10 uM EDTA | 95% H2O/5% D2O | 41 mM | 7 | AMBIENT atm | 293.15 | Bruker AVANCE 750 |
2 | 3D CBCA(CO)NH | 0.5 mM [U-13C; U-15N] Tsr2(1-152), 0.5 mM S26A(100-119), 20 mM sodium phosphate, 1 mM [U-2H] DTT, 10 uM EDTA | 95% H2O/5% D2O | 41 mM | 7 | AMBIENT atm | 293.15 | Bruker AVANCE 750 |
3 | 3D HBHA(CO)NH | 0.5 mM [U-13C; U-15N] Tsr2(1-152), 0.5 mM S26A(100-119), 20 mM sodium phosphate, 1 mM [U-2H] DTT, 10 uM EDTA | 95% H2O/5% D2O | 41 mM | 7 | AMBIENT atm | 293.15 | Bruker AVANCE 750 |
4 | 3D HCCH-TOCSY | 0.5 mM [U-13C; U-15N] Tsr2(1-152), 0.5 mM S26A(100-119), 20 mM sodium phosphate, 1 mM [U-2H] DTT, 10 uM EDTA | 95% H2O/5% D2O | 41 mM | 7 | AMBIENT atm | 293.15 | Bruker AVANCE 600 |
5 | 3D 1H-15N NOESY | 0.5 mM [U-13C; U-15N] Tsr2(1-152), 0.5 mM S26A(100-119), 20 mM sodium phosphate, 1 mM [U-2H] DTT, 10 uM EDTA | 95% H2O/5% D2O | 41 mM | 7 | AMBIENT atm | 293.15 | Bruker AVANCE 900 |
6 | 3D 1H-13C NOESY aliphatic | 0.5 mM [U-13C; U-15N] Tsr2(1-152), 0.5 mM S26A(100-119), 20 mM sodium phosphate, 1 mM [U-2H] DTT, 10 uM EDTA | 95% H2O/5% D2O | 41 mM | 7 | AMBIENT atm | 293.15 | Bruker AVANCE 900 |
7 | 3D 1H-13C NOESY aromatic | 0.5 mM [U-13C; U-15N] Tsr2(1-152), 0.5 mM S26A(100-119), 20 mM sodium phosphate, 1 mM [U-2H] DTT, 10 uM EDTA | 95% H2O/5% D2O | 41 mM | 7 | AMBIENT atm | 293.15 | Bruker AVANCE 900 |
8 | 3D HNCA | 0.5 mM Tsr2(1-152), 0.5 mM [U-13C; U-15N] S26A(100-119), 20 mM sodium phosphate, 1 mM [U-2H] DTT, 10 uM EDTA | 95% H2O/5% D2O | 41 mM | 7 | AMBIENT atm | 293.15 | Bruker AVANCE 700 |
9 | 3D CBCA(CO)NH | 0.5 mM Tsr2(1-152), 0.5 mM [U-13C; U-15N] S26A(100-119), 20 mM sodium phosphate, 1 mM [U-2H] DTT, 10 uM EDTA | 95% H2O/5% D2O | 41 mM | 7 | AMBIENT atm | 293.15 | Bruker AVANCE 700 |
10 | 3D C(CO)NH | 0.5 mM Tsr2(1-152), 0.5 mM [U-13C; U-15N] S26A(100-119), 20 mM sodium phosphate, 1 mM [U-2H] DTT, 10 uM EDTA | 95% H2O/5% D2O | 41 mM | 7 | AMBIENT atm | 293.15 | Bruker AVANCE 600 |
11 | 3D H(CCO)NH | 0.5 mM Tsr2(1-152), 0.5 mM [U-13C; U-15N] S26A(100-119), 20 mM sodium phosphate, 1 mM [U-2H] DTT, 10 uM EDTA | 95% H2O/5% D2O | 41 mM | 7 | AMBIENT atm | 293.15 | Bruker AVANCE 600 |
12 | 3D 1H-15N NOESY | 0.5 mM Tsr2(1-152), 0.5 mM [U-13C; U-15N] S26A(100-119), 20 mM sodium phosphate, 1 mM [U-2H] DTT, 10 uM EDTA | 95% H2O/5% D2O | 41 mM | 7 | AMBIENT atm | 293.15 | Bruker AVANCE 900 |
13 | 3D 1H-13C NOESY aliphatic | 0.5 mM Tsr2(1-152), 0.5 mM [U-13C; U-15N] S26A(100-119), 20 mM sodium phosphate, 1 mM [U-2H] DTT, 10 uM EDTA | 95% H2O/5% D2O | 41 mM | 7 | AMBIENT atm | 293.15 | Bruker AVANCE 900 |
14 | 3D 1H-13C NOESY aromatic | 0.5 mM Tsr2(1-152), 0.5 mM [U-13C; U-15N] S26A(100-119), 20 mM sodium phosphate, 1 mM [U-2H] DTT, 10 uM EDTA | 95% H2O/5% D2O | 41 mM | 7 | AMBIENT atm | 293.15 | Bruker AVANCE 900 |