NMR Experiment |
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Experiment | Type | Sample Contents | Solvent | Ionic Strength | pH | Pressure | Temperature (K) | Spectrometer |
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1 | 2D 1H-15N HSQC | 400 uM [U-100% 13C; U-100% 15N] NleG5-1, 15 mM HEPES, 250 mM sodium chloride, 5 mM L-arginine, 5 mM sodium citrate, 1 mM benzamidine, 10 uM DTT, 0.01 % w/v sodium azide | 90% H2O/10% D2O | 400 mM | 7.5 | 1 atm | 303 | Bruker AVANCE 500 |
2 | 2D 1H-13C HSQC | 400 uM [U-100% 13C; U-100% 15N] NleG5-1, 15 mM HEPES, 250 mM sodium chloride, 5 mM L-arginine, 5 mM sodium citrate, 1 mM benzamidine, 10 uM DTT, 0.01 % w/v sodium azide | 90% H2O/10% D2O | 400 mM | 7.5 | 1 atm | 303 | Bruker AVANCE 500 |
3 | 3D HNCA | 400 uM [U-100% 13C; U-100% 15N] NleG5-1, 15 mM HEPES, 250 mM sodium chloride, 5 mM L-arginine, 5 mM sodium citrate, 1 mM benzamidine, 10 uM DTT, 0.01 % w/v sodium azide | 90% H2O/10% D2O | 400 mM | 7.5 | 1 atm | 303 | Bruker AVANCE 500 |
4 | 3D HNCO | 400 uM [U-100% 13C; U-100% 15N] NleG5-1, 15 mM HEPES, 250 mM sodium chloride, 5 mM L-arginine, 5 mM sodium citrate, 1 mM benzamidine, 10 uM DTT, 0.01 % w/v sodium azide | 90% H2O/10% D2O | 400 mM | 7.5 | 1 atm | 303 | Bruker AVANCE 500 |
5 | 3D CBCA(CO)NH | 400 uM [U-100% 13C; U-100% 15N] NleG5-1, 15 mM HEPES, 250 mM sodium chloride, 5 mM L-arginine, 5 mM sodium citrate, 1 mM benzamidine, 10 uM DTT, 0.01 % w/v sodium azide | 90% H2O/10% D2O | 400 mM | 7.5 | 1 atm | 303 | Bruker AVANCE 500 |
9 | 3D HBHA(CO)NH | 400 uM [U-100% 13C; U-100% 15N] NleG5-1, 15 mM HEPES, 250 mM sodium chloride, 5 mM L-arginine, 5 mM sodium citrate, 1 mM benzamidine, 10 uM DTT, 0.01 % w/v sodium azide | 90% H2O/10% D2O | 400 mM | 7.5 | 1 atm | 303 | Bruker AVANCE 500 |
8 | 3D 1H-13C NOESY aliphatic | 400 uM [U-100% 13C; U-100% 15N] NleG5-1, 15 mM HEPES, 250 mM sodium chloride, 5 mM L-arginine, 5 mM sodium citrate, 1 mM benzamidine, 10 uM DTT, 0.01 % w/v sodium azide | 90% H2O/10% D2O | 400 mM | 7.5 | 1 atm | 303 | Bruker AVANCE II 800 |
7 | 3D 1H-13C NOESY aromatic | 400 uM [U-100% 13C; U-100% 15N] NleG5-1, 15 mM HEPES, 250 mM sodium chloride, 5 mM L-arginine, 5 mM sodium citrate, 1 mM benzamidine, 10 uM DTT, 0.01 % w/v sodium azide | 90% H2O/10% D2O | 400 mM | 7.5 | 1 atm | 303 | Bruker AVANCE II 800 |
6 | 3D 1H-15N NOESY | 400 uM [U-100% 13C; U-100% 15N] NleG5-1, 15 mM HEPES, 250 mM sodium chloride, 5 mM L-arginine, 5 mM sodium citrate, 1 mM benzamidine, 10 uM DTT, 0.01 % w/v sodium azide | 90% H2O/10% D2O | 400 mM | 7.5 | 1 atm | 303 | Bruker AVANCE II 800 |
12 | 3D 1H-13C NOESY aromatic | 400 uM [U-100% 13C; U-100% 15N] NleG5-1, 15 mM HEPES, 250 mM sodium chloride, 5 mM L-arginine, 5 mM sodium citrate, 1 mM benzamidine, 10 uM DTT, 0.01 % w/v sodium azide | 90% H2O/10% D2O | 400 mM | 7.5 | 1 atm | 303 | Bruker AVANCE II 800 |
10 | 3D HCCH-TOCSY | 400 uM [U-100% 13C; U-100% 15N] NleG5-1, 15 mM HEPES, 250 mM sodium chloride, 5 mM L-arginine, 5 mM sodium citrate, 1 mM benzamidine, 10 uM DTT, 0.01 % w/v sodium azide | 90% H2O/10% D2O | 400 mM | 7.5 | 1 atm | 303 | Bruker AVANCE II 800 |
11 | 3D CCH-TOCSY | 400 uM [U-100% 13C; U-100% 15N] NleG5-1, 15 mM HEPES, 250 mM sodium chloride, 5 mM L-arginine, 5 mM sodium citrate, 1 mM benzamidine, 10 uM DTT, 0.01 % w/v sodium azide | 90% H2O/10% D2O | 400 mM | 7.5 | 1 atm | 303 | Bruker AVANCE II 800 |