NMR Experiment |
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Experiment | Type | Sample Contents | Solvent | Ionic Strength | pH | Pressure | Temperature (K) | Spectrometer |
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1 | 2D 1H-15N HSQC | 0.3 mM [U-95% 13C; U-95% 15N] 26S proteasome regulatory subunit Rpn5, 10 % v/v [U-99% 2H] D2O, 0.1 mg/mL DSS, 1 mM DTT, 50 mM sodium phosphate, 50 mM sodium sulfate, 2 % v/v trifluoroethanol, 5 % w/v sucrose | 90% H2O/10% D2O | 50 mM sodium phosphate buffer, 50 mM Na2SO4 mM | 6.0 | ambient Pa | 298 | Bruker AVANCE III 500 |
2 | 2D 1H-13C HSQC | 0.3 mM [U-95% 13C; U-95% 15N] 26S proteasome regulatory subunit Rpn5, 10 % v/v [U-99% 2H] D2O, 0.1 mg/mL DSS, 1 mM DTT, 50 mM sodium phosphate, 50 mM sodium sulfate, 2 % v/v trifluoroethanol, 5 % w/v sucrose | 90% H2O/10% D2O | 50 mM sodium phosphate buffer, 50 mM Na2SO4 mM | 6.0 | ambient Pa | 298 | Bruker AVANCE III 500 |
3 | 3D HNCA | 0.3 mM [U-95% 13C; U-95% 15N] 26S proteasome regulatory subunit Rpn5, 10 % v/v [U-99% 2H] D2O, 0.1 mg/mL DSS, 1 mM DTT, 50 mM sodium phosphate, 50 mM sodium sulfate, 2 % v/v trifluoroethanol, 5 % w/v sucrose | 90% H2O/10% D2O | 50 mM sodium phosphate buffer, 50 mM Na2SO4 mM | 6.0 | ambient Pa | 298 | Bruker AVANCE III 500 |
4 | 3D HN(CO)CA | 0.3 mM [U-95% 13C; U-95% 15N] 26S proteasome regulatory subunit Rpn5, 10 % v/v [U-99% 2H] D2O, 0.1 mg/mL DSS, 1 mM DTT, 50 mM sodium phosphate, 50 mM sodium sulfate, 2 % v/v trifluoroethanol, 5 % w/v sucrose | 90% H2O/10% D2O | 50 mM sodium phosphate buffer, 50 mM Na2SO4 mM | 6.0 | ambient Pa | 298 | Bruker AVANCE III 500 |
5 | 3D HNCO | 0.3 mM [U-95% 13C; U-95% 15N] 26S proteasome regulatory subunit Rpn5, 10 % v/v [U-99% 2H] D2O, 0.1 mg/mL DSS, 1 mM DTT, 50 mM sodium phosphate, 50 mM sodium sulfate, 2 % v/v trifluoroethanol, 5 % w/v sucrose | 90% H2O/10% D2O | 50 mM sodium phosphate buffer, 50 mM Na2SO4 mM | 6.0 | ambient Pa | 298 | Bruker AVANCE III 500 |
7 | 3D HN(CA)CO | 0.3 mM [U-95% 13C; U-95% 15N] 26S proteasome regulatory subunit Rpn5, 10 % v/v [U-99% 2H] D2O, 0.1 mg/mL DSS, 1 mM DTT, 50 mM sodium phosphate, 50 mM sodium sulfate, 2 % v/v trifluoroethanol, 5 % w/v sucrose | 90% H2O/10% D2O | 50 mM sodium phosphate buffer, 50 mM Na2SO4 mM | 6.0 | ambient Pa | 298 | Bruker AVANCE III 500 |
6 | 3D HNCACB | 0.3 mM [U-95% 13C; U-95% 15N] 26S proteasome regulatory subunit Rpn5, 10 % v/v [U-99% 2H] D2O, 0.1 mg/mL DSS, 1 mM DTT, 50 mM sodium phosphate, 50 mM sodium sulfate, 2 % v/v trifluoroethanol, 5 % w/v sucrose | 90% H2O/10% D2O | 50 mM sodium phosphate buffer, 50 mM Na2SO4 mM | 6.0 | ambient Pa | 298 | Bruker AVANCE III 500 |
8 | 3D CBCA(CO)NH | 0.3 mM [U-95% 13C; U-95% 15N] 26S proteasome regulatory subunit Rpn5, 10 % v/v [U-99% 2H] D2O, 0.1 mg/mL DSS, 1 mM DTT, 50 mM sodium phosphate, 50 mM sodium sulfate, 2 % v/v trifluoroethanol, 5 % w/v sucrose | 90% H2O/10% D2O | 50 mM sodium phosphate buffer, 50 mM Na2SO4 mM | 6.0 | ambient Pa | 298 | Bruker AVANCE III 500 |
10 | 3D HBHA(CO)NH | 0.3 mM [U-95% 13C; U-95% 15N] 26S proteasome regulatory subunit Rpn5, 10 % v/v [U-99% 2H] D2O, 0.1 mg/mL DSS, 1 mM DTT, 50 mM sodium phosphate, 50 mM sodium sulfate, 2 % v/v trifluoroethanol, 5 % w/v sucrose | 90% H2O/10% D2O | 50 mM sodium phosphate buffer, 50 mM Na2SO4 mM | 6.0 | ambient Pa | 298 | Bruker AVANCE III 500 |
9 | 3D HCCH-COSY | 0.3 mM [U-95% 13C; U-95% 15N] 26S proteasome regulatory subunit Rpn5, 10 % v/v [U-99% 2H] D2O, 0.1 mg/mL DSS, 1 mM DTT, 50 mM sodium phosphate, 50 mM sodium sulfate, 2 % v/v trifluoroethanol, 5 % w/v sucrose | 90% H2O/10% D2O | 50 mM sodium phosphate buffer, 50 mM Na2SO4 mM | 6.0 | ambient Pa | 298 | Bruker AVANCE III 500 |
11 | 3D HCCH-COSY | 0.3 mM [U-95% 13C; U-95% 15N] 26S proteasome regulatory subunit Rpn5, 10 % v/v [U-99% 2H] D2O, 0.1 mg/mL DSS, 1 mM DTT, 50 mM sodium phosphate, 50 mM sodium sulfate, 2 % v/v trifluoroethanol, 5 % w/v sucrose | 90% H2O/10% D2O | 50 mM sodium phosphate buffer, 50 mM Na2SO4 mM | 6.0 | ambient Pa | 298 | Bruker AVANCE III 500 |
12 | 3D 15N-edited TOCSY-HSQC | 0.3 mM [U-95% 13C; U-95% 15N] 26S proteasome regulatory subunit Rpn5, 10 % v/v [U-99% 2H] D2O, 0.1 mg/mL DSS, 1 mM DTT, 50 mM sodium phosphate, 50 mM sodium sulfate, 2 % v/v trifluoroethanol, 5 % w/v sucrose | 90% H2O/10% D2O | 50 mM sodium phosphate buffer, 50 mM Na2SO4 mM | 6.0 | ambient Pa | 298 | Bruker AVANCE III 500 |
13 | 3D 1H-15N NOESY | 0.3 mM [U-95% 13C; U-95% 15N] 26S proteasome regulatory subunit Rpn5, 10 % v/v [U-99% 2H] D2O, 0.1 mg/mL DSS, 1 mM DTT, 50 mM sodium phosphate, 50 mM sodium sulfate, 2 % v/v trifluoroethanol, 5 % w/v sucrose | 90% H2O/10% D2O | 50 mM sodium phosphate buffer, 50 mM Na2SO4 mM | 6.0 | ambient Pa | 298 | Bruker AVANCE III 950 |
14 | 3D 1H-15C NOESY | 0.3 mM [U-95% 13C; U-95% 15N] 26S proteasome regulatory subunit Rpn5, 10 % v/v [U-99% 2H] D2O, 0.1 mg/mL DSS, 1 mM DTT, 50 mM sodium phosphate, 50 mM sodium sulfate, 2 % v/v trifluoroethanol, 5 % w/v sucrose | 90% H2O/10% D2O | 50 mM sodium phosphate buffer, 50 mM Na2SO4 mM | 6.0 | ambient Pa | 298 | Bruker AVANCE III 950 |