5FJ5

Structure of the in vitro assembled bacteriophage phi6 polymerase complex


ELECTRON MICROSCOPY
Sample
BACTERIOPHAGE PHI6 POLYMERASE COMPLEX ASSEMBLED IN VITRO FROM PURIFIED PROTEINS P1, P2, AND P4
Specimen Preparation
Sample Aggregation StatePARTICLE
Vitrification InstrumentFEI VITROBOT MARK IV
Cryogen NameETHANE
Sample Vitrification DetailsVITRIFICATION 1 -- CRYOGEN- ETHANE, TEMPERATURE- 120, INSTRUMENT- FEI VITROBOT MARK IV, METHOD- BLOT 4 SECONDS BEFORE PLUNGING,
3D Reconstruction
Reconstruction MethodSINGLE PARTICLE
Number of Particles4379
Reported Resolution (Å)4.8
Resolution Method
Other DetailsSUBMISSION BASED ON EXPERIMENTAL DATA FROM EMDB EMD-3185. (DEPOSITION ID: 13852).
Refinement Type
Symmetry TypePOINT
Point SymmetryI
Map-Model Fitting and Refinement
Id1 (4K7H)
Refinement SpaceREAL
Refinement ProtocolRIGID BODY FIT
Refinement Target
Overall B Value
Fitting Procedure
DetailsMETHOD--RIGID BODY REFINEMENT PROTOCOL--X-RAY
Data Acquisition
Detector TypeGATAN K2 (4k x 4k)
Electron Dose (electrons/Å**2)16
Imaging Experiment1
Date of Experiment2014-06-12
Temperature (Kelvin)81
Microscope ModelFEI TECNAI F30
Minimum Defocus (nm)1100
Maximum Defocus (nm)2600
Minimum Tilt Angle (degrees)
Maximum Tilt Angle (degrees)
Nominal CS2
Imaging ModeBRIGHT FIELD
Specimen Holder Model
Nominal Magnification160000
Calibrated Magnification37037
SourceFIELD EMISSION GUN
Acceleration Voltage (kV)300
Imaging DetailsDOSE RATE 6-8 E- PER PIX PER SEC
EM Software
TaskSoftware PackageVersion
MODEL FITTINGCoot
MODEL FITTINGPHENIX
MODEL FITTINGUCSF Chimera
RECONSTRUCTIONRELION
Image Processing
CTF Correction TypeCTF Correction DetailsNumber of Particles SelectedParticle Selection Details
EACH PARTICLE