5A8H

cryo-ET subtomogram averaging of BG505 SOSIP.664 in complex with sCD4, 17b, and 8ANC195


ELECTRON MICROSCOPY
Sample
SOLUBLE HIV-1 ENV TRIMER BG505 SOSIP.664 IN COMPLEX WITH SOLUBLE CD4S (D1-D2), BROADLY NEUTRALIZING ANTIBODY 17B FABS, AND BROADLY NEUTRALIZING ANTIBODY 8ANC195 VARIANT G32K5 FABS
Specimen Preparation
Sample Aggregation StatePARTICLE
Vitrification InstrumentFEI VITROBOT MARK IV
Cryogen NameETHANE-PROPANE
Sample Vitrification DetailsVITRIFICATION 1 -- CRYOGEN- ETHANE-PROPANE MIXTURE, HUMIDITY- 95, TEMPERATURE- 70, INSTRUMENT- FEI
3D Reconstruction
Reconstruction MethodTOMOGRAPHY
Number of Particles1745
Reported Resolution (Å)23
Resolution Method
Other DetailsSUBTOMOGRAM AVERAGING SUBMISSION BASED ON EXPERIMENTAL DATA FROM EMDB EMD-3096. (DEPOSITION ID: 13596).
Refinement Type
Symmetry TypePOINT
Point SymmetryC1
Map-Model Fitting and Refinement
Id1 (1RZK)
Refinement SpaceREAL
Refinement ProtocolRIGID BODY FIT
Refinement Target
Overall B Value
Fitting Procedure
DetailsMETHOD--RIGID BODY
Data Acquisition
Detector TypeGATAN K2 (4k x 4k)
Electron Dose (electrons/Å**2)120
Imaging Experiment1
Date of Experiment2015-01-25
Temperature (Kelvin)
Microscope ModelFEI TECNAI F30
Minimum Defocus (nm)
Maximum Defocus (nm)
Minimum Tilt Angle (degrees)-60
Maximum Tilt Angle (degrees)60
Nominal CS2.2
Imaging ModeBRIGHT FIELD
Specimen Holder Model
Nominal Magnification41000
Calibrated Magnification
SourceFIELD EMISSION GUN
Acceleration Voltage (kV)300
Imaging Details
EM Software
TaskSoftware PackageVersion
MODEL FITTINGUCSF Chimera
RECONSTRUCTIONEMAN2
RECONSTRUCTIONIMOD
RECONSTRUCTIONPEET
Image Processing
CTF Correction TypeCTF Correction DetailsNumber of Particles SelectedParticle Selection Details
EACH MICROGRAPH