4UIH

Cryo-EM structure of Dengue virus serotype 2 strain New Guinea-C complexed with human antibody 2D22 Fab at 37 degree C. The Fab molecules were added to the virus before 37 degree C incubation.


ELECTRON MICROSCOPY
Sample
DENGUE VIRUS SEROTYPE 2 STRAIN NEW GUINEA-C COMPLEXED WITH FAB FRAGMENTS OF HUMAN ANTIBODY 2D22 AT 37 DEGREE C
Specimen Preparation
Sample Aggregation StatePARTICLE
Vitrification InstrumentFEI VITROBOT MARK IV
Cryogen NameETHANE
Sample Vitrification DetailsVITRIFICATION 1 -- CRYOGEN- ETHANE, HUMIDITY- 100, INSTRUMENT- FEI VITROBOT MARK IV, METHOD- BLOTTED WITH FILTER PAPER FOR 2 SECONDS PRIOR TO SNAP FRE ...VITRIFICATION 1 -- CRYOGEN- ETHANE, HUMIDITY- 100, INSTRUMENT- FEI VITROBOT MARK IV, METHOD- BLOTTED WITH FILTER PAPER FOR 2 SECONDS PRIOR TO SNAP FREEZING,
3D Reconstruction
Reconstruction MethodSINGLE PARTICLE
Number of Particles4288
Reported Resolution (Å)20
Resolution Method
Other DetailsSUBMISSION BASED ON EXPERIMENTAL DATA FROM EMDB EMD-2968. (DEPOSITION ID: 13278).
Refinement Type
Symmetry TypePOINT
Point SymmetryI
Map-Model Fitting and Refinement
Id1 (4UIF)
Refinement Space
Refinement ProtocolOTHER
Refinement Target
Overall B Value
Fitting Procedure
DetailsMETHOD--MANUAL FITTING USING O PROGRAM REFINEMENT PROTOCOL--CRYO-EM
Data Acquisition
Detector TypeFEI FALCON II (4k x 4k)
Electron Dose (electrons/Å**2)20
Imaging Experiment1
Date of Experiment2014-06-13
Temperature (Kelvin)100
Microscope ModelFEI TITAN KRIOS
Minimum Defocus (nm)1000
Maximum Defocus (nm)3600
Minimum Tilt Angle (degrees)
Maximum Tilt Angle (degrees)
Nominal CS2.7
Imaging ModeBRIGHT FIELD
Specimen Holder Model
Nominal Magnification47000
Calibrated Magnification
SourceFIELD EMISSION GUN
Acceleration Voltage (kV)300
Imaging Details
EM Software
TaskSoftware PackageVersion
MODEL FITTINGO
RECONSTRUCTIONEMAN1
RECONSTRUCTIONEMAN2
RECONSTRUCTIONMPSA
Image Processing
CTF Correction TypeCTF Correction DetailsNumber of Particles SelectedParticle Selection Details
EACH PARTICLE