4BOM

Structure of herpesvirus fusion glycoprotein B-bilayer complex revealing the protein-membrane and lateral protein-protein interaction


ELECTRON MICROSCOPY
Sample
HUMAN HERPESVIRUS 1 ENVELOPED GLYCOPROTEIN GB ECTODOMAIN LACKING THE MEMBRANE PROXIMAL REGION BOUND TO LIPOSOMES
Specimen Preparation
Sample Aggregation StatePARTICLE
Vitrification Instrument
Cryogen NameETHANE-PROPANE
Sample Vitrification DetailsLIQUID NITROGEN PROPANE MIXTURE
3D Reconstruction
Reconstruction MethodTOMOGRAPHY
Number of Particles786
Reported Resolution (Å)27
Resolution Method
Other DetailsTHE MISSING FUSION LOOP RESIDUES 261 AND 262 WERE MODELED IN ALL THE THREE CHAINS OF USING MODELLER BASED ON THE GB CRYSTAL STRUCTURE (PDB ID 2GUM) SU ...THE MISSING FUSION LOOP RESIDUES 261 AND 262 WERE MODELED IN ALL THE THREE CHAINS OF USING MODELLER BASED ON THE GB CRYSTAL STRUCTURE (PDB ID 2GUM) SUBMISSION BASED ON EXPERIMENTAL DATA FROM EMDB EMD-2380. (DEPOSITION ID: 11670).
Refinement Type
Symmetry TypePOINT
Point SymmetryC3
Map-Model Fitting and Refinement
Id1 (3NWF)
Refinement SpaceREAL
Refinement ProtocolFLEXIBLE FIT
Refinement Target
Overall B Value
Fitting Procedure
DetailsMETHOD--FLEXIBLE REFINEMENT PROTOCOL--FLEXIBLE
Data Acquisition
Detector TypeGATAN ULTRASCAN 4000 (4k x 4k)
Electron Dose (electrons/Å**2)100
Imaging Experiment1
Date of Experiment2008-01-17
Temperature (Kelvin)
Microscope ModelFEI TECNAI F20
Minimum Defocus (nm)2000
Maximum Defocus (nm)2000
Minimum Tilt Angle (degrees)-60
Maximum Tilt Angle (degrees)60
Nominal CS2
Imaging ModeBRIGHT FIELD
Specimen Holder Model
Nominal Magnification
Calibrated Magnification67000
SourceFIELD EMISSION GUN
Acceleration Voltage (kV)200
Imaging Details
EM Software
TaskSoftware PackageVersion
MODEL FITTINGFlex-EM
RECONSTRUCTIONJSUBTOMO
Image Processing
CTF Correction TypeCTF Correction DetailsNumber of Particles SelectedParticle Selection Details
LOW PASS FILTER TO THE FIRST ZERO CROSSING OF THE CTF