4AU6

Location of the dsRNA-dependent polymerase, VP1, in rotavirus particles

ELECTRON MICROSCOPY

Sample
ROTAVIRUS DLP

Specimen Preparation
Sample Aggregation State	PARTICLE
Vitrification Instrument	HOMEMADE PLUNGER
Cryogen Name	ETHANE
Sample Vitrification Details	LIQUID ETHANE

3D Reconstruction
Reconstruction Method	SINGLE PARTICLE
Number of Particles	7000
Reported Resolution (Å)	6
Resolution Method
Other Details	SUBMISSION BASED ON EXPERIMENTAL DATA FROM EMDB EMD-2100.(DEPOSITION ID: 10810).
Refinement Type
Symmetry Type	POINT
Point Symmetry	I

Map-Model Fitting and Refinement
Id	1 (2R7O)
Refinement Space	RECIPROCAL
Refinement Protocol	OTHER
Refinement Target	Cross-correlation coefficient
Overall B Value
Fitting Procedure
Details	METHOD--URO REFINEMENT PROTOCOL--X-RAY

Data Acquisition
Detector Type	KODAK SO-163 FILM
Electron Dose (electrons/Å**2)	15

Imaging Experiment	1
Date of Experiment	2007-06-01
Temperature (Kelvin)	90
Microscope Model	FEI TECNAI F30
Minimum Defocus (nm)	1100
Maximum Defocus (nm)	3500
Minimum Tilt Angle (degrees)
Maximum Tilt Angle (degrees)	0.001
Nominal CS	2
Imaging Mode	BRIGHT FIELD
Specimen Holder Model
Nominal Magnification	59000
Calibrated Magnification	56540
Source	FIELD EMISSION GUN
Acceleration Voltage (kV)	300
Imaging Details

EM Software
Task	Software Package	Version
MODEL FITTING	URO
MODEL FITTING	VEDA
RECONSTRUCTION	FPM
RECONSTRUCTION	RICO

Image Processing
CTF Correction Type	CTF Correction Details	Number of Particles Selected	Particle Selection Details
	INDIVIDUAL PARTICLE PHASE FLIPPING

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.