3ZFS
Cryo-EM structure of the F420-reducing NiFe-hydrogenase from a methanogenic archaeon with bound substrate
ELECTRON MICROSCOPY
Sample |
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F420-REDUCING HYDROGENASE |
Specimen Preparation | |
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Sample Aggregation State | PARTICLE |
Vitrification Instrument | FEI VITROBOT MARK I |
Cryogen Name | ETHANE |
Sample Vitrification Details | LIQUID ETHANE |
3D Reconstruction | |
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Reconstruction Method | SINGLE PARTICLE |
Number of Particles | 97290 |
Reported Resolution (Å) | 4 |
Resolution Method | |
Other Details | SUBMISSION BASED ON EXPERIMENTAL DATA FROM EMDB EMD-2097.(DEPOSITION ID: 10788). |
Refinement Type | |
Symmetry Type | POINT |
Point Symmetry | T |
Map-Model Fitting and Refinement | |||||
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Id | 1 (2WPN) | ||||
Refinement Space | REAL | ||||
Refinement Protocol | RIGID BODY FIT | ||||
Refinement Target | |||||
Overall B Value | |||||
Fitting Procedure | |||||
Details | REFINEMENT PROTOCOL--RIGID BODY |
Data Acquisition | |||||||||
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Detector Type | KODAK SO-163 FILM | ||||||||
Electron Dose (electrons/Å**2) | 15 |
Imaging Experiment | 1 |
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Date of Experiment | 2011-02-11 |
Temperature (Kelvin) | 77 |
Microscope Model | FEI POLARA 300 |
Minimum Defocus (nm) | 1650 |
Maximum Defocus (nm) | 3820 |
Minimum Tilt Angle (degrees) | |
Maximum Tilt Angle (degrees) | |
Nominal CS | 2 |
Imaging Mode | BRIGHT FIELD |
Specimen Holder Model | |
Nominal Magnification | 59000 |
Calibrated Magnification | 61400 |
Source | FIELD EMISSION GUN |
Acceleration Voltage (kV) | 200 |
Imaging Details |
EM Software | ||
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Task | Software Package | Version |
MODEL FITTING | Coot | |
MODEL FITTING | UCSF Chimera | |
RECONSTRUCTION | EMAN | 2 |
Image Processing | ||||
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CTF Correction Type | CTF Correction Details | Number of Particles Selected | Particle Selection Details | |
PER MICROGRAPH |