Cryo-EM reconstruction of quasi-HPV16 complex with H263.A2 Fab
ELECTRON MICROSCOPY
Sample
quasi-HPV16 complexed with H263.A2 Fabs
Sample Components
Human papillomavirus 16
H263.A2 Fab
Specimen Preparation
Sample Aggregation State
PARTICLE
Vitrification Instrument
GATAN CRYOPLUNGE 3
Cryogen Name
ETHANE
Sample Vitrification Details
Plunged into liquid ethane (GATAN CRYOPLUNGE 3).
3D Reconstruction
Reconstruction Method
SINGLE PARTICLE
Number of Particles
8908
Reported Resolution (Å)
13
Resolution Method
FSC 0.5 CUT-OFF
Other Details
Semi-automatic particle selection was performed using e2boxer.py to obtain the particle coordinates, followed by particle boxing, linearization, norma ...
Semi-automatic particle selection was performed using e2boxer.py to obtain the particle coordinates, followed by particle boxing, linearization, normalization, and apodization of the images using Robem. Defocus and astigmatism values used to perform contrast transfer function (CTF) correction were assessed using Robem for the extracted particles. The icosahedrally averaged reconstruction was initiated using a random model generated with setup_rmc and reached 14 A resolution estimated at a Fourier Shell Correlation (FSC) of 0.5. For the last step of refinement, the final maps were CTF-corrected using a B factor of 200 A2. (Single particle details: The particles were selected using semi-automatic program e2boxer.py (EMAN2)) (Single particle--Applied symmetry: I)
