3J22

The Enterovirus 71 A-particle

ELECTRON MICROSCOPY

Sample
Human Enterovirus 71 A-Particle

Specimen Preparation
Sample Aggregation State	PARTICLE
Vitrification Instrument	FEI VITROBOT MARK III
Cryogen Name	OTHER
Sample Vitrification Details	ETHANE-PROPANE MIXTURE

3D Reconstruction
Reconstruction Method	SINGLE PARTICLE
Number of Particles	11436
Reported Resolution (Å)	6.3
Resolution Method	FSC 0.5 CUT-OFF
Other Details	A single data set was used for the reconstruction. Particles were selected using an automatic selection program (EMAN).
Refinement Type
Symmetry Type	POINT
Point Symmetry	I

Map-Model Fitting and Refinement
Id	1 (3VBU)
Refinement Space	REAL
Refinement Protocol	RIGID BODY FIT
Refinement Target	correlation coefficient
Overall B Value
Fitting Procedure
Details	METHOD--local refinement REFINEMENT PROTOCOL--rigid body DETAILS--One protomer was fit in Chimera, used to generate a full crystal map, then docked a ...	METHOD--local refinement REFINEMENT PROTOCOL--rigid body DETAILS--One protomer was fit in Chimera, used to generate a full crystal map, then docked as a rigid body using Situs into the cryoEM density map

Data Acquisition
Detector Type	KODAK SO-163 FILM
Electron Dose (electrons/Å**2)	15

Imaging Experiment	1
Date of Experiment	2012-04-17
Temperature (Kelvin)	95
Microscope Model	FEI TECNAI F20
Minimum Defocus (nm)	1730
Maximum Defocus (nm)	4280
Minimum Tilt Angle (degrees)
Maximum Tilt Angle (degrees)
Nominal CS	2
Imaging Mode	BRIGHT FIELD
Specimen Holder Model	GATAN LIQUID NITROGEN
Nominal Magnification	50000
Calibrated Magnification	50000
Source	FIELD EMISSION GUN
Acceleration Voltage (kV)	200
Imaging Details

EM Software
Task	Software Package	Version
MODEL FITTING	Situs
MODEL FITTING	UCSF Chimera
RECONSTRUCTION	Auto3DEM
RECONSTRUCTION	EMAN	2

Image Processing
CTF Correction Type	CTF Correction Details	Number of Particles Selected	Particle Selection Details
	auto3DEM

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.