NMR Experiment |
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Experiment | Type | Sample Contents | Solvent | Ionic Strength | pH | Pressure | Temperature (K) | Spectrometer |
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1 | 2D NOESY | 1.5mM unlabelled Bcl-xL/Beclin-1 complex in 99.9% D2O; 50mM potassium phosphate | 99.9% D2O | 50mM potassium phosphate | 7.0 | 1 atm | 303 | |
2 | 3D_15N-separated_NOESY | 1.5mM uniformly 15N labelled Bcl-xL/Beclin-1 complex in 90% H2O/10% D2O; 50mM potassium phosphate | 90% H2O/10% D2O | 50mM potassium phosphate | 7.0 | 1 atm | 303 | |
3 | 3D_13C-separated_NOESY | 1.5mM uniformly 15N/13C labelled Bcl-xL/Beclin-1 complex in 99.9% D2O; 50mM potassium phosphate | 99.9% D2O | 50mM potassium phosphate | 7.0 | 1 atm | 303 | |
4 | HNCO, HNCA, HN(CO)CA, HNCACB and CBCA(CO)NH | 1.5mM 80% deuterated, uniformly 15N/13C labelled Bcl-xL/Beclin-1 complex in 90% H2O/10% D2O; 50mM potassium phosphate | 90% H2O/10% D2O | 50mM potassium phosphate | 7.0 | 1 atm | 303 | |
5 | 3D_15N-separated_NOESY | 1.5mM 100% deuterated, uniformly 15N labelled Bcl-xL/Beclin-1 complex in 90% H2O/10% D2O; 50mM potassium phosphate | 90% H2O/10% D2O | 50mM potassium phosphate | 7.0 | 1 atm | 303 | |