2OF6
Structure of immature West Nile virus
ELECTRON MICROSCOPY
| Sample |
|---|
| immature West Nile |
| Specimen Preparation | |
|---|---|
| Sample Aggregation State | PARTICLE |
| Vitrification Instrument | |
| Cryogen Name | ETHANE |
| Sample Vitrification Details | rapidly frozen by plunging into an ethane slush |
| 3D Reconstruction | |
|---|---|
| Reconstruction Method | SINGLE PARTICLE |
| Number of Particles | 341 |
| Reported Resolution (Å) | 24 |
| Resolution Method | |
| Other Details | |
| Refinement Type | |
| Symmetry Type | POINT |
| Point Symmetry | I |
| Map-Model Fitting and Refinement | |||||
|---|---|---|---|---|---|
| Id | 1 (2HG0) | ||||
| Refinement Space | REAL | ||||
| Refinement Protocol | RIGID BODY FIT | ||||
| Refinement Target | best fit using criterial described in paper by Rossmann et al., Journal of structural biology 136, 190-200 (2001) | ||||
| Overall B Value | |||||
| Fitting Procedure | |||||
| Details | METHOD--Three E molecules were fitted consecutively REFINEMENT PROTOCOL--rigid body | ||||
| Data Acquisition | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detector Type | KODAK SO-163 FILM | ||||||||
| Electron Dose (electrons/Å**2) | 22 | ||||||||
| Imaging Experiment | 1 |
|---|---|
| Date of Experiment | 2006-03-15 |
| Temperature (Kelvin) | 87 |
| Microscope Model | FEI/PHILIPS CM300FEG/T |
| Minimum Defocus (nm) | 1300 |
| Maximum Defocus (nm) | 3100 |
| Minimum Tilt Angle (degrees) | |
| Maximum Tilt Angle (degrees) | |
| Nominal CS | 2 |
| Imaging Mode | BRIGHT FIELD |
| Specimen Holder Model | |
| Nominal Magnification | 45000 |
| Calibrated Magnification | 47400 |
| Source | FIELD EMISSION GUN |
| Acceleration Voltage (kV) | 300 |
| Imaging Details |
| EM Software | ||
|---|---|---|
| Task | Software Package | Version |
| MODEL FITTING | EMfit | |
| RECONSTRUCTION | EM3DR | |
| RECONSTRUCTION | PFT | |
| RECONSTRUCTION | POR | |
| Image Processing | ||||
|---|---|---|---|---|
| CTF Correction Type | CTF Correction Details | Number of Particles Selected | Particle Selection Details | |
| CTF correction of each particle | ||||
