NMR Experiment |
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Experiment | Type | Sample Contents | Solvent | Ionic Strength | pH | Pressure | Temperature (K) | Spectrometer |
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1 | 2D 1H-15N TROSY | 0.15-0.20 mM [U-100% 15N; U-85% 2H] sNTD, 10 mM sodium phosphate, 100 mM sodium chloride, 0.005 % Tween 20, 0.5 mM TCEP, 0.01 % sodium azide, 0.5 M [U-98% 2H] Choline o-sulfate | 95% H2O/5% D2O | | 7.3 | ambient | 298 | |
2 | 3D HNCA-TROSY | 0.15-0.20 mM [U-100% 13C; U-100% 15N; U-85% 2H] sNTD, 10 mM sodium phosphate, 100 mM sodium chloride, 0.005 % Tween 20, 0.5 mM TCEP, 0.01 % sodium azide, 0.5 M [U-98% 2H] Choline o-sulfate | 95% H2O/5% D2O | | 7.3 | ambient | 298 | |
3 | 3D HN(CO)CA-TROSY | 0.15-0.20 mM [U-100% 13C; U-100% 15N; U-85% 2H] sNTD, 10 mM sodium phosphate, 100 mM sodium chloride, 0.005 % Tween 20, 0.5 mM TCEP, 0.01 % sodium azide, 0.5 M [U-98% 2H] Choline o-sulfate | 95% H2O/5% D2O | | 7.3 | ambient | 298 | |
4 | 3D HNCO-TROSY | 0.15-0.20 mM [U-100% 13C; U-100% 15N; U-85% 2H] sNTD, 10 mM sodium phosphate, 100 mM sodium chloride, 0.005 % Tween 20, 0.5 mM TCEP, 0.01 % sodium azide, 0.5 M [U-98% 2H] Choline o-sulfate | 95% H2O/5% D2O | | 7.3 | ambient | 298 | |
5 | 3D HN(CA)CO-TROSY | 0.15-0.20 mM [U-100% 13C; U-100% 15N; U-85% 2H] sNTD, 10 mM sodium phosphate, 100 mM sodium chloride, 0.005 % Tween 20, 0.5 mM TCEP, 0.01 % sodium azide, 0.5 M [U-98% 2H] Choline o-sulfate | 95% H2O/5% D2O | | 7.3 | ambient | 298 | |
6 | 3D HNCACB-TROSY | 0.15-0.20 mM [U-100% 13C; U-100% 15N; U-85% 2H] sNTD, 10 mM sodium phosphate, 100 mM sodium chloride, 0.005 % Tween 20, 0.5 mM TCEP, 0.01 % sodium azide, 0.5 M [U-98% 2H] Choline o-sulfate | 95% H2O/5% D2O | | 7.3 | ambient | 298 | |
7 | 3D HN(CO)CACB-TROSY | 0.15-0.20 mM [U-100% 13C; U-100% 15N; U-85% 2H] sNTD, 10 mM sodium phosphate, 100 mM sodium chloride, 0.005 % Tween 20, 0.5 mM TCEP, 0.01 % sodium azide, 0.5 M [U-98% 2H] Choline o-sulfate | 95% H2O/5% D2O | | 7.3 | ambient | 298 | |
8 | 3D 15N-edited NOESY | 0.05-0.10 mM [U-100% 13C; U-100% 15N; U-60% 2H] sNTD, 10 mM sodium phosphate, 100 mM sodium chloride, 0.005 % Tween 20, 0.5 mM TCEP, 0.01 % sodium azide, 0.5 M [U-98% 2H] Choline o-sulfate | 95% H2O/5% D2O | | 7.3 | ambient | 298 | |
9 | 2D 1H-15N TROSY | 0.05-0.10 mM [U-80% 2H; 100% 15N-Arg] sNTD, 10 mM sodium phosphate, 100 mM sodium chloride, 0.005 % Tween 20, 0.5 mM TCEP, 0.01 % sodium azide, 0.5 M [U-98% 2H] Choline o-sulfate | 95% H2O/5% D2O | | 7.3 | ambient | 298 | |
10 | 2D 1H-15N TROSY | 0.05-0.10 mM [U-80% 2H; 100% 15N-Ile] sNTD, 10 mM sodium phosphate, 100 mM sodium chloride, 0.005 % Tween 20, 0.5 mM TCEP, 0.01 % sodium azide, 0.5 M [U-98% 2H] Choline o-sulfate | 95% H2O/5% D2O | | 7.3 | ambient | 298 | |
11 | 2D 1H-15N TROSY | 0.05-0.10 mM [U-80% 2H; 100% 15N-Leu] sNTD, 10 mM sodium phosphate, 100 mM sodium chloride, 0.005 % Tween 20, 0.5 mM TCEP, 0.01 % sodium azide, 0.5 M [U-98% 2H] Choline o-sulfate | 95% H2O/5% D2O | | 7.3 | ambient | 298 | |
12 | 2D 1H-15N TROSY | 0.05-0.10 mM [U-80% 2H; 100% 15N-Lys] sNTD, 10 mM sodium phosphate, 100 mM sodium chloride, 0.005 % Tween 20, 0.5 mM TCEP, 0.01 % sodium azide, 0.5 M [U-98% 2H] Choline o-sulfate | 95% H2O/5% D2O | | 7.3 | ambient | 298 | |
13 | 2D 1H-15N TROSY | 0.05-0.10 mM [U-80% 2H; 100% 15N-Phe] sNTD, 10 mM sodium phosphate, 100 mM sodium chloride, 0.005 % Tween 20, 0.5 mM TCEP, 0.01 % sodium azide, 0.5 M [U-98% 2H] Choline o-sulfate | 95% H2O/5% D2O | | 7.3 | ambient | 298 | |
14 | 2D 1H-15N TROSY | 0.05-0.10 mM [U-80% 2H; 100% 15N-Tyr] sNTD, 10 mM sodium phosphate, 100 mM sodium chloride, 0.005 % Tween 20, 0.5 mM TCEP, 0.01 % sodium azide, 0.5 M [U-98% 2H] Choline o-sulfate | 95% H2O/5% D2O | | 7.3 | ambient | 298 | |
15 | 2D 1H-13C HSQC | 0.05-0.10 mM [U-80% 2H; 100% 1H, 100% 13C-Ala] sNTD, 10 mM sodium phosphate, 100 mM sodium chloride, 0.005 % Tween 20, 0.5 mM TCEP, 0.01 % sodium azide, 0.5 M [U-98% 2H] Choline o-sulfate | 100% D2O | | 7.3 | ambient | 298 | |
16 | 2D 1H-13C HSQC | 0.05-0.10 mM [U-80% 2H; 100% 1H, 100% 13C-Arg] sNTD, 10 mM sodium phosphate, 100 mM sodium chloride, 0.005 % Tween 20, 0.5 mM TCEP, 0.01 % sodium azide, 0.5 M [U-98% 2H] Choline o-sulfate | 100% D2O | | 7.3 | ambient | 298 | |
17 | 2D 1H-13C HSQC | 0.05-0.10 mM [U-80% 2H; 100% 1H, 100% 13C-Ile] sNTD, 10 mM sodium phosphate, 100 mM sodium chloride, 0.005 % Tween 20, 0.5 mM TCEP, 0.01 % sodium azide, 0.5 M [U-98% 2H] Choline o-sulfate | 100% D2O | | 7.3 | ambient | 298 | |
18 | 2D 1H-13C HSQC | 0.05-0.10 mM [U-80% 2H; 100% 1H, 100% 13C-Leu] sNTD, 10 mM sodium phosphate, 100 mM sodium chloride, 0.005 % Tween 20, 0.5 mM TCEP, 0.01 % sodium azide, 0.5 M [U-98% 2H] Choline o-sulfate | 100% D2O | | 7.3 | ambient | 298 | |
19 | 2D 1H-13C HSQC | 0.05-0.10 mM [U-80% 2H; 100% 1H, 100% 13C-Lys] sNTD, 10 mM sodium phosphate, 100 mM sodium chloride, 0.005 % Tween 20, 0.5 mM TCEP, 0.01 % sodium azide, 0.5 M [U-98% 2H] Choline o-sulfate | 100% D2O | | 7.3 | ambient | 298 | |
20 | 2D 1H-13C HSQC | 0.05-0.10 mM [U-80% 2H; 100% 1H, 100% 13C-Met] sNTD, 10 mM sodium phosphate, 100 mM sodium chloride, 0.005 % Tween 20, 0.5 mM TCEP, 0.01 % sodium azide, 0.5 M [U-98% 2H] Choline o-sulfate | 100% D2O | | 7.3 | ambient | 298 | |
21 | 2D 1H-13C HSQC | 0.05-0.10 mM [U-80% 2H; 100% 1H, 100% 13C-Phe] sNTD, 10 mM sodium phosphate, 100 mM sodium chloride, 0.005 % Tween 20, 0.5 mM TCEP, 0.01 % sodium azide, 0.5 M [U-98% 2H] Choline o-sulfate | 100% D2O | | 7.3 | ambient | 298 | |
22 | 2D 1H-13C HSQC | 0.05-0.10 mM [U-80% 2H; 100% 1H, 100% 13C-Thr] sNTD, 10 mM sodium phosphate, 100 mM sodium chloride, 0.005 % Tween 20, 0.5 mM TCEP, 0.01 % sodium azide, 0.5 M [U-98% 2H] Choline o-sulfate | 100% D2O | | 7.3 | ambient | 298 | |
23 | 2D 1H-13C HSQC | 0.05-0.10 mM [U-80% 2H; 100% 1H, 100% 13C-Tyr] sNTD, 10 mM sodium phosphate, 100 mM sodium chloride, 0.005 % Tween 20, 0.5 mM TCEP, 0.01 % sodium azide, 0.5 M [U-98% 2H] Choline o-sulfate | 100% D2O | | 7.3 | ambient | 298 | |
24 | 2D 1H-13C HSQC | 0.05-0.10 mM [U-80% 2H; 100% 1H, 100% 13C-Val] sNTD, 10 mM sodium phosphate, 100 mM sodium chloride, 0.005 % Tween 20, 0.5 mM TCEP, 0.01 % sodium azide, 0.5 M [U-98% 2H] Choline o-sulfate | 100% D2O | | 7.3 | ambient | 298 | |
25 | 2D 1H-1H NOESY | 0.05-0.10 mM [U-80% 2H; 100% 1H, 100% 13C-Ala] sNTD, 10 mM sodium phosphate, 100 mM sodium chloride, 0.005 % Tween 20, 0.5 mM TCEP, 0.01 % sodium azide, 0.5 M [U-98% 2H] Choline o-sulfate | 100% D2O | | 7.3 | ambient | 298 | |
26 | 2D 1H-1H NOESY | 0.05-0.10 mM [U-80% 2H; 100% 1H, 100% 13C-Arg] sNTD, 10 mM sodium phosphate, 100 mM sodium chloride, 0.005 % Tween 20, 0.5 mM TCEP, 0.01 % sodium azide, 0.5 M [U-98% 2H] Choline o-sulfate | 100% D2O | | 7.3 | ambient | 298 | |
27 | 2D 1H-1H NOESY | 0.05-0.10 mM [U-80% 2H; 100% 1H, 100% 13C-Ile] sNTD, 10 mM sodium phosphate, 100 mM sodium chloride, 0.005 % Tween 20, 0.5 mM TCEP, 0.01 % sodium azide, 0.5 M [U-98% 2H] Choline o-sulfate | 100% D2O | | 7.3 | ambient | 298 | |
28 | 2D 1H-1H NOESY | 0.05-0.10 mM [U-80% 2H; 100% 1H, 100% 13C-Leu] sNTD, 10 mM sodium phosphate, 100 mM sodium chloride, 0.005 % Tween 20, 0.5 mM TCEP, 0.01 % sodium azide, 0.5 M [U-98% 2H] Choline o-sulfate | 100% D2O | | 7.3 | ambient | 298 | |
29 | 2D 1H-1H NOESY | 0.05-0.10 mM [U-80% 2H; 100% 1H, 100% 13C-Lys] sNTD, 10 mM sodium phosphate, 100 mM sodium chloride, 0.005 % Tween 20, 0.5 mM TCEP, 0.01 % sodium azide, 0.5 M [U-98% 2H] Choline o-sulfate | 100% D2O | | 7.3 | ambient | 298 | |
30 | 2D 1H-1H NOESY | 0.05-0.10 mM [U-80% 2H; 100% 1H, 100% 13C-Met] sNTD, 10 mM sodium phosphate, 100 mM sodium chloride, 0.005 % Tween 20, 0.5 mM TCEP, 0.01 % sodium azide, 0.5 M [U-98% 2H] Choline o-sulfate | 100% D2O | | 7.3 | ambient | 298 | |
31 | 2D 1H-1H NOESY | 0.05-0.10 mM [U-80% 2H; 100% 1H, 100% 13C-Phe] sNTD, 10 mM sodium phosphate, 100 mM sodium chloride, 0.005 % Tween 20, 0.5 mM TCEP, 0.01 % sodium azide, 0.5 M [U-98% 2H] Choline o-sulfate | 100% D2O | | 7.3 | ambient | 298 | |
32 | 2D 1H-1H NOESY | 0.05-0.10 mM [U-80% 2H; 100% 1H, 100% 13C-Thr] sNTD, 10 mM sodium phosphate, 100 mM sodium chloride, 0.005 % Tween 20, 0.5 mM TCEP, 0.01 % sodium azide, 0.5 M [U-98% 2H] Choline o-sulfate | 100% D2O | | 7.3 | ambient | 298 | |
33 | 2D 1H-1H NOESY | 0.05-0.10 mM [U-80% 2H; 100% 1H, 100% 13C-Tyr] sNTD, 10 mM sodium phosphate, 100 mM sodium chloride, 0.005 % Tween 20, 0.5 mM TCEP, 0.01 % sodium azide, 0.5 M [U-98% 2H] Choline o-sulfate | 100% D2O | | 7.3 | ambient | 298 | |
34 | 2D 1H-1H NOESY | 0.05-0.10 mM [U-80% 2H; 100% 1H, 100% 13C-Val] sNTD, 10 mM sodium phosphate, 100 mM sodium chloride, 0.005 % Tween 20, 0.5 mM TCEP, 0.01 % sodium azide, 0.5 M [U-98% 2H] Choline o-sulfate | 100% D2O | | 7.3 | ambient | 298 | |
35 | 2D 1H-1H NOESY | 0.05-0.10 mM [U-80% 2H; 100% 1H-Phe,Tyr,Ile] sNTD, 10 mM sodium phosphate, 100 mM sodium chloride, 0.005 % Tween 20, 0.5 mM TCEP, 0.01 % sodium azide, 0.5 M [U-98% 2H] Choline o-sulfate | 100% D2O | | 7.3 | ambient | 298 | |
36 | 2D 1H-1H NOESY | 0.05-0.10 mM [U-80% 2H; 100% 1H-Phe,Tyr,Leu] sNTD, 10 mM sodium phosphate, 100 mM sodium chloride, 0.005 % Tween 20, 0.5 mM TCEP, 0.01 % sodium azide, 0.5 M [U-98% 2H] Choline o-sulfate | 100% D2O | | 7.3 | ambient | 298 | |
37 | 2D 1H-1H NOESY | 0.05-0.10 mM [U-80% 2H; 100% 1H-Phe,Tyr,Val] sNTD, 10 mM sodium phosphate, 100 mM sodium chloride, 0.005 % Tween 20, 0.5 mM TCEP, 0.01 % sodium azide, 0.5 M [U-98% 2H] Choline o-sulfate | 100% D2O | | 7.3 | ambient | 298 | |
38 | 2D 1H-1H NOESY | 0.05-0.10 mM [U-80% 2H; 100% 1H-Trp,Ile,Leu,Val] sNTD, 10 mM sodium phosphate, 100 mM sodium chloride, 0.005 % Tween 20, 0.5 mM TCEP, 0.01 % sodium azide, 0.5 M [U-98% 2H] Choline o-sulfate | 100% D2O | | 7.3 | ambient | 298 | |
39 | 2D 1H-1H NOESY | 0.15-0.20 mM sNTD, 10 mM sodium phosphate, 100 mM sodium chloride, 0.005 % Tween 20, 0.5 mM TCEP, 0.01 % sodium azide, 0.5 M [U-98% 2H] Choline o-sulfate | 100% D2O | | 7.3 | ambient | 298 | |
40 | 3D 1H-13C NOESY | 0.05-0.10 mM [U-100% 13C; U-100% 15N; U-60% 2H] sNTD, 10 mM sodium phosphate, 100 mM sodium chloride, 0.005 % Tween 20, 0.5 mM TCEP, 0.01 % sodium azide, 0.5 M [U-98% 2H] Choline o-sulfate | 95% H2O/5% D2O | | 7.3 | ambient | 298 | |