Experiment: 2MT6

SOLUTION NMR

NMR Experiment
Experiment	Type	Sample Contents	Solvent	Ionic Strength	pH	Pressure	Temperature (K)	Spectrometer
1	3D HNCO	200-400 uM [U-100% 13C; U-100% 15N] Ube2w	90% H2O/10% D2O	150	7.0	ambient	298
2	3D HNCA	200-400 uM [U-100% 13C; U-100% 15N] Ube2w	90% H2O/10% D2O	150	7.0	ambient	298
3	3D HN(CO)CA	200-400 uM [U-100% 13C; U-100% 15N] Ube2w	90% H2O/10% D2O	150	7.0	ambient	298
4	3D HNCACB	200-400 uM [U-100% 13C; U-100% 15N] Ube2w	90% H2O/10% D2O	150	7.0	ambient	298
5	3D CBCA(CO)NH	200-400 uM [U-100% 13C; U-100% 15N] Ube2w	90% H2O/10% D2O	150	7.0	ambient	298
6	2D 1H-15N HSQC	200-400 uM [U-100% 13C; U-100% 15N] Ube2w	90% H2O/10% D2O	150	7.0	ambient	298

NMR Spectrometer Information
Spectrometer	Manufacturer	Model	Field Strength
1	Bruker	DMX	500
2	Bruker	INOVA	600

NMR Ensemble Information
Conformer Selection Criteria	structures with the lowest energy
Conformers Calculated Total Number	16000
Conformers Submitted Total Number	20
Representative Model	1 (closest to the average)

Computation: NMR Software
#	Classification	Version	Software Name	Author
1	structure solution	CS-ROSETTA		Shen, Vernon, Baker and Bax
2	refinement	CS-ROSETTA		Shen, Vernon, Baker and Bax

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.