NMR Experiment |
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Experiment | Type | Sample Contents | Solvent | Ionic Strength | pH | Pressure | Temperature (K) | Spectrometer |
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1 | 2D 1H-15N HSQC | 0.4-0.6 mM [U-100% 15N] CPEB4RRM12-9, 100 mM sodium chloride-10, 50 mM sodium phosphate-11, 1 mM DTT-12 | 90% H2O/10% D2O | 0.15 | 7.0 | ambient | 303 | |
2 | 2D TROSY | 0.4-0.6 mM [U-100% 15N] CPEB4RRM12-9, 100 mM sodium chloride-10, 50 mM sodium phosphate-11, 1 mM DTT-12 | 90% H2O/10% D2O | 0.15 | 7.0 | ambient | 303 | |
3 | 2D 1H-13C HSQC | 0.4-0.6 mM [U-100% 13C; U-100% 15N] CPEB4RRM12-1, 100 mM sodium chloride-2, 50 mM sodium phosphate-3, 1 mM DTT-4 | 90% H2O/10% D2O | 0.15 | 7.0 | ambient | 303 | |
4 | 2D 1H-13C HSQC aliphatic | 0.4-0.6 mM [U-100% 13C; U-100% 15N] CPEB4RRM12-1, 100 mM sodium chloride-2, 50 mM sodium phosphate-3, 1 mM DTT-4 | 90% H2O/10% D2O | 0.15 | 7.0 | ambient | 303 | |
5 | 2D 1H-13C HSQC aromatic | 0.4-0.6 mM [U-100% 13C; U-100% 15N] CPEB4RRM12-1, 100 mM sodium chloride-2, 50 mM sodium phosphate-3, 1 mM DTT-4 | 90% H2O/10% D2O | 0.15 | 7.0 | ambient | 303 | |
6 | 2D 1H-1H TOCSY | 0.4-0.6 mM [U-100% 15N] CPEB4RRM12-13, 100 mM sodium chloride-14, 50 mM sodium phosphate-15, 1 mM DTT-16 | 100% D2O | 0.15 | 7.0 | ambient | 303 | |
7 | 2D 1H-1H NOESY | 0.4-0.6 mM [U-100% 15N] CPEB4RRM12-13, 100 mM sodium chloride-14, 50 mM sodium phosphate-15, 1 mM DTT-16 | 100% D2O | 0.15 | 7.0 | ambient | 303 | |
8 | 3D CBCA(CO)NH | 0.4-0.6 mM [U-100% 13C; U-100% 15N; U-80% 2H] CPEB4RRM12-5, 100 mM sodium chloride-6, 50 mM sodium phosphate-7, 1 mM DTT-8 | 90% H2O/10% D2O | 0.15 | 7.0 | ambient | 303 | |
9 | 3D trHNCACB | 0.4-0.6 mM [U-100% 13C; U-100% 15N; U-80% 2H] CPEB4RRM12-5, 100 mM sodium chloride-6, 50 mM sodium phosphate-7, 1 mM DTT-8 | 90% H2O/10% D2O | 0.15 | 7.0 | ambient | 303 | |
10 | 3D trHNCA | 0.4-0.6 mM [U-100% 13C; U-100% 15N; U-80% 2H] CPEB4RRM12-5, 100 mM sodium chloride-6, 50 mM sodium phosphate-7, 1 mM DTT-8 | 90% H2O/10% D2O | 0.15 | 7.0 | ambient | 303 | |
11 | 3D trHN(CO)CA | 0.4-0.6 mM [U-100% 13C; U-100% 15N; U-80% 2H] CPEB4RRM12-5, 100 mM sodium chloride-6, 50 mM sodium phosphate-7, 1 mM DTT-8 | 90% H2O/10% D2O | 0.15 | 7.0 | ambient | 303 | |
12 | 3D trHNCO | 0.4-0.6 mM [U-100% 15N] CPEB4RRM12-9, 100 mM sodium chloride-10, 50 mM sodium phosphate-11, 1 mM DTT-12 | 90% H2O/10% D2O | 0.15 | 7.0 | ambient | 303 | |
13 | 3D trHCACO | 0.4-0.6 mM [U-100% 13C; U-100% 15N; U-80% 2H] CPEB4RRM12-5, 100 mM sodium chloride-6, 50 mM sodium phosphate-7, 1 mM DTT-8 | 90% H2O/10% D2O | 0.15 | 7.0 | ambient | 303 | |
14 | 3D HCCH-TOCSY | 0.4-0.6 mM [U-100% 13C; U-100% 15N] CPEB4RRM12-1, 100 mM sodium chloride-2, 50 mM sodium phosphate-3, 1 mM DTT-4 | 90% H2O/10% D2O | 0.15 | 7.0 | ambient | 303 | |
15 | 3D HCCH-TOCSY | 0.4-0.6 mM [U-100% 13C; U-100% 15N] CPEB4RRM12-1, 100 mM sodium chloride-2, 50 mM sodium phosphate-3, 1 mM DTT-4 | 90% H2O/10% D2O | 0.15 | 7.0 | ambient | 303 | |
16 | 3D 1H-15N NOESY | 0.4-0.6 mM [U-100% 13C; U-100% 15N; U-80% 2H] CPEB4RRM12-5, 100 mM sodium chloride-6, 50 mM sodium phosphate-7, 1 mM DTT-8 | 90% H2O/10% D2O | 0.15 | 7.0 | ambient | 303 | |
17 | 3D 1H-13C NOESY aliphatic | 0.4-0.6 mM [U-100% 13C; U-100% 15N] CPEB4RRM12-1, 100 mM sodium chloride-2, 50 mM sodium phosphate-3, 1 mM DTT-4 | 90% H2O/10% D2O | 0.15 | 7.0 | ambient | 303 | |
18 | 3D 1H-13C NOESY aromatic | 0.4-0.6 mM [U-100% 13C; U-100% 15N] CPEB4RRM12-1, 100 mM sodium chloride-2, 50 mM sodium phosphate-3, 1 mM DTT-4 | 90% H2O/10% D2O | 0.15 | 7.0 | ambient | 303 | |