2LNL
Structure of human CXCR1 in phospholipid bilayers
SOLID-STATE NMR
| NMR Experiment | ||||||||
|---|---|---|---|---|---|---|---|---|
| Experiment | Type | Sample Contents | Solvent | Ionic Strength | pH | Pressure | Temperature (K) | Spectrometer |
| 1 | NCACX | 2-4 mg [U-100% 13C; U-100% 15N] CXCR1 | 100% H2O | 20 | 7 | ambient | 298 | |
| 2 | SLF | 2-4 mg [U-100% 13C; U-100% 15N] CXCR1 | 100% H2O | 20 | 7 | ambient | 298 | |
| 3 | PDSD | 2-4 mg [U-100% 13C; U-100% 15N] CXCR1 | 100% H2O | 20 | 7 | ambient | 298 | |
| NMR Spectrometer Information | |||
|---|---|---|---|
| Spectrometer | Manufacturer | Model | Field Strength |
| 1 | Bruker | Avance | 750 |
| NMR Refinement | ||
|---|---|---|
| Method | Details | Software |
| simulated annealing | THE ANI COORDINATES HAVE BEEN INCLUDED IN THE RESTRAINT FILE. THE ANI Z AXIS SPECIFIES THE DIRECTION OF THE NORMAL TO THE PLANE OF THE LIPID BILAYER MEMBRANE. | X-PLOR_NIH |
| NMR Ensemble Information | |
|---|---|
| Conformer Selection Criteria | structures with the lowest energy |
| Conformers Calculated Total Number | 100 |
| Conformers Submitted Total Number | 10 |
| Representative Model | 1 (lowest energy) |
| Computation: NMR Software | ||||
|---|---|---|---|---|
| # | Classification | Version | Software Name | Author |
| 1 | refinement | X-PLOR_NIH | Schwieters, Kuszewski, Tjandra and Clore | |
