2L46
C-terminal zinc finger of the HIVNCp7 with platinated DNA
SOLUTION NMR
| NMR Experiment | ||||||||
|---|---|---|---|---|---|---|---|---|
| Experiment | Type | Sample Contents | Solvent | Ionic Strength | pH | Pressure | Temperature (K) | Spectrometer |
| 1 | 2D 1H-1H TOCSY | 1 mM C-terminal zinc knuckle of the HIVNCp7, 1 mM DNA | 90% H2O/10% D2O | 6.0 | 5.7 | ambient | 298 | |
| 2 | 2D DQF-COSY | 1 mM C-terminal zinc knuckle of the HIVNCp7, 1 mM DNA | 90% H2O/10% D2O | 6.0 | 5.7 | ambient | 298 | |
| 3 | 2D 1H-1H NOESY | 1 mM C-terminal zinc knuckle of the HIVNCp7, 1 mM DNA | 90% H2O/10% D2O | 6.0 | 5.7 | ambient | 298 | |
| 4 | 2D 1H-15N HSQC | 1 mM C-terminal zinc knuckle of the HIVNCp7, 1 mM DNA | 90% H2O/10% D2O | 6.0 | 5.7 | ambient | 298 | |
| NMR Spectrometer Information | |||
|---|---|---|---|
| Spectrometer | Manufacturer | Model | Field Strength |
| 1 | Bruker | AVANCE | 600 |
| NMR Refinement | ||
|---|---|---|
| Method | Details | Software |
| DGSA-distance geometry simulated annealing | CYANA | |
| NMR Ensemble Information | |
|---|---|
| Conformer Selection Criteria | target function |
| Conformers Calculated Total Number | 21 |
| Conformers Submitted Total Number | 20 |
| Representative Model | 1 (lowest energy) |
| Computation: NMR Software | ||||
|---|---|---|---|---|
| # | Classification | Version | Software Name | Author |
| 1 | refinement | CYANA | 2.1 | Guntert, Mumenthaler and Wuthrich |
| 2 | peak picking | Sparky | 3.114 | Goddard |
| 3 | chemical shift assignment | Sparky | 3.114 | Goddard |
