NMR Experiment |
---|
Experiment | Type | Sample Contents | Solvent | Ionic Strength | pH | Pressure | Temperature (K) | Spectrometer |
---|
1 | 2D 1H-15N HSQC | 0.8 mM [U-100% 13C; U-100% 15N; U-100% 2H] SR10, 10 mM DTT, 0.02 % sodium azide, 5 mM calcium chloride, 100 mM sodium chloride, 1 x protease inhibitor, 20 mM MES, 50 uM DSS | 90% H2O/10% D2O | | 6.5 | ambient | 298 | |
2 | 2D 1H-13C HSQC | 0.8 mM [U-100% 13C; U-100% 15N; U-100% 2H] SR10, 10 mM DTT, 0.02 % sodium azide, 5 mM calcium chloride, 100 mM sodium chloride, 1 x protease inhibitor, 20 mM MES, 50 uM DSS | 90% H2O/10% D2O | | 6.5 | ambient | 298 | |
3 | 3D HNCO | 0.8 mM [U-100% 13C; U-100% 15N; U-100% 2H] SR10, 10 mM DTT, 0.02 % sodium azide, 5 mM calcium chloride, 100 mM sodium chloride, 1 x protease inhibitor, 20 mM MES, 50 uM DSS | 90% H2O/10% D2O | | 6.5 | ambient | 298 | |
4 | 3D HNCA | 0.8 mM [U-100% 13C; U-100% 15N; U-100% 2H] SR10, 10 mM DTT, 0.02 % sodium azide, 5 mM calcium chloride, 100 mM sodium chloride, 1 x protease inhibitor, 20 mM MES, 50 uM DSS | 90% H2O/10% D2O | | 6.5 | ambient | 298 | |
5 | 3D TRHNCACB 2H dec | 0.8 mM [U-100% 13C; U-100% 15N; U-100% 2H] SR10, 10 mM DTT, 0.02 % sodium azide, 5 mM calcium chloride, 100 mM sodium chloride, 1 x protease inhibitor, 20 mM MES, 50 uM DSS | 90% H2O/10% D2O | | 6.5 | ambient | 298 | |
6 | 3D HN(CA)CO | 0.8 mM [U-100% 13C; U-100% 15N; U-100% 2H] SR10, 10 mM DTT, 0.02 % sodium azide, 5 mM calcium chloride, 100 mM sodium chloride, 1 x protease inhibitor, 20 mM MES, 50 uM DSS | 90% H2O/10% D2O | | 6.5 | ambient | 298 | |
7 | 2D 1H-15N HSQC - T1 | 0.8 mM [U-100% 13C; U-100% 15N; U-100% 2H] SR10, 10 mM DTT, 0.02 % sodium azide, 5 mM calcium chloride, 100 mM sodium chloride, 1 x protease inhibitor, 20 mM MES, 50 uM DSS | 90% H2O/10% D2O | | 6.5 | ambient | 298 | |
8 | 2D 1H-15N HSQC - T2 | 0.8 mM [U-100% 13C; U-100% 15N; U-100% 2H] SR10, 10 mM DTT, 0.02 % sodium azide, 5 mM calcium chloride, 100 mM sodium chloride, 1 x protease inhibitor, 20 mM MES, 50 uM DSS | 90% H2O/10% D2O | | 6.5 | ambient | 298 | |
9 | 3D 1H-15N NOESY | 0.8 mM [U-100% 13C; U-100% 15N; U-100% 2H] SR10, 10 mM DTT, 0.02 % sodium azide, 5 mM calcium chloride, 100 mM sodium chloride, 1 x protease inhibitor, 20 mM MES, 50 uM DSS | 90% H2O/10% D2O | | 6.5 | ambient | 298 | |
10 | 3D 1H-13C NOESY | 0.8 mM [U-100% 13C; U-100% 15N; U-100% 2H] SR10, 10 mM DTT, 0.02 % sodium azide, 5 mM calcium chloride, 100 mM sodium chloride, 1 x protease inhibitor, 20 mM MES, 50 uM DSS | 90% H2O/10% D2O | | 6.5 | ambient | 298 | |
11 | 3D (N15)HSQC-NOESY-(N15)HSQC | 0.8 mM [U-100% 13C; U-100% 15N; U-100% 2H] SR10, 10 mM DTT, 0.02 % sodium azide, 5 mM calcium chloride, 100 mM sodium chloride, 1 x protease inhibitor, 20 mM MES, 50 uM DSS | 90% H2O/10% D2O | | 6.5 | ambient | 298 | |
12 | 3D (C13)HSQC-NOESY-(C13)HSQC | 0.8 mM [U-100% 13C; U-100% 15N; U-100% 2H] SR10, 10 mM DTT, 0.02 % sodium azide, 5 mM calcium chloride, 100 mM sodium chloride, 1 x protease inhibitor, 20 mM MES, 50 uM DSS | 90% H2O/10% D2O | | 6.5 | ambient | 298 | |
13 | 3D (N15)HSQC-NOESY-(C13)HSQC | 0.8 mM [U-100% 13C; U-100% 15N; U-100% 2H] SR10, 10 mM DTT, 0.02 % sodium azide, 5 mM calcium chloride, 100 mM sodium chloride, 1 x protease inhibitor, 20 mM MES, 50 uM DSS | 90% H2O/10% D2O | | 6.5 | ambient | 298 | |
14 | 3D (C13)HSQC-NOESY-(N15)HSQC | 0.8 mM [U-100% 13C; U-100% 15N; U-100% 2H] SR10, 10 mM DTT, 0.02 % sodium azide, 5 mM calcium chloride, 100 mM sodium chloride, 1 x protease inhibitor, 20 mM MES, 50 uM DSS | 90% H2O/10% D2O | | 6.5 | ambient | 298 | |
15 | 2D 1H-13C HSQC | 1.2 mM [U-5% 13C; U-100% 15N] SR10, 10 mM DTT, 0.02 % sodium azide, 5 mM calcium chloride, 100 mM sodium chloride, 1 x protease inhibitor, 20 mM MES, 50 uM DSS | 90% H2O/10% D2O | | 6.5 | ambient | 298 | |
16 | 2D 1H-15N TROSY | 1.2 mM [U-5% 13C; U-100% 15N] SR10, 10 mM DTT, 0.02 % sodium azide, 5 mM calcium chloride, 200 mM sodium chloride, 1 x protease inhibitor, 20 mM MES, 50 uM DSS | 90% H2O/10% D2O | | 6.5 | ambient | 298 | |