NMR Experiment |
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Experiment | Type | Sample Contents | Solvent | Ionic Strength | pH | Pressure | Temperature (K) | Spectrometer |
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1 | 2D 1H-1H NOESY | 1 mM RNA (5'-R(*GP*AP*CP*GP*AP*GP*CP*GP*UP*CP*A)-3')-1, 80 mM sodium chloride-2, 10 mM sodium phosphate-3, 0.5 mM EDTA-4 | 90% H2O/10% D2O | 0.1 | 6.1 | ambient | 273 | |
2 | 2D 1H-13C HSQC | 1 mM RNA (5'-R(*GP*AP*CP*GP*AP*GP*CP*GP*UP*CP*A)-3')-1, 80 mM sodium chloride-2, 10 mM sodium phosphate-3, 0.5 mM EDTA-4 | 90% H2O/10% D2O | 0.1 | 6.1 | ambient | 273 | |
3 | 2D 1H-1H TOCSY | 1 mM RNA (5'-R(*GP*AP*CP*GP*AP*GP*CP*GP*UP*CP*A)-3')-5, 80 mM sodium chloride-6, 10 mM sodium phosphate-7, 0.5 mM EDTA-8 | 100% D2O | 0.1 | 6.1 | ambient | 288 | |
4 | 2D DQF-COSY | 1 mM RNA (5'-R(*GP*AP*CP*GP*AP*GP*CP*GP*UP*CP*A)-3')-5, 80 mM sodium chloride-6, 10 mM sodium phosphate-7, 0.5 mM EDTA-8 | 100% D2O | 0.1 | 6.1 | ambient | 288 | |
5 | 2D 1H-31P HETCOR | 1 mM RNA (5'-R(*GP*AP*CP*GP*AP*GP*CP*GP*UP*CP*A)-3')-5, 80 mM sodium chloride-6, 10 mM sodium phosphate-7, 0.5 mM EDTA-8 | 100% D2O | 0.1 | 6.1 | ambient | 288 | |
6 | 2D 1H-1H NOESY | 1 mM RNA (5'-R(*GP*AP*CP*GP*AP*GP*CP*GP*UP*CP*A)-3')-5, 80 mM sodium chloride-6, 10 mM sodium phosphate-7, 0.5 mM EDTA-8 | 100% D2O | 0.1 | 6.1 | ambient | 288 | |