NMR Experiment |
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Experiment | Type | Sample Contents | Solvent | Ionic Strength | pH | Pressure | Temperature (K) | Spectrometer |
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1 | 2D 1H-15N HSQC | 0.5 mM [U-100% 15N] 1655-1822, 2 mM F3, 50 mM sodium chloride, 20 mM sodium phosphate, 2 mM DTT | 90% H2O/10% D2O | 50 | 6.5 | ambient | 298 | |
2 | 2D 1H-15N HSQC | 0.5 mM 1655-1822, 2 mM [U-100% 15N] F3, 50 mM sodium chloride, 20 mM sodium phosphate, 2 mM DTT | 90% H2O/10% D2O | 50 | 6.5 | ambient | 298 | |
3 | 3D 1H-13C NOESY | 0.5 mM [U-100% 15N] 1655-1822, 2 mM F3, 50 mM sodium chloride, 20 mM sodium phosphate, 2 mM DTT | 90% H2O/10% D2O | 50 | 6.5 | ambient | 298 | |
4 | 3D 1H-13C NOESY | 0.5 mM 1655-1822, 2 mM [U-100% 15N] F3, 50 mM sodium chloride, 20 mM sodium phosphate, 2 mM DTT | 90% H2O/10% D2O | 50 | 6.5 | ambient | 298 | |
5 | F1-filtered, F3-edited NOESY | 0.5 mM [U-100% 15N] 1655-1822, 2 mM F3, 50 mM sodium chloride, 20 mM sodium phosphate, 2 mM DTT | 90% H2O/10% D2O | 50 | 6.5 | ambient | 298 | |
6 | F1-filtered, F3-edited NOESY | 0.5 mM 1655-1822, 2 mM [U-100% 15N] F3, 50 mM sodium chloride, 20 mM sodium phosphate, 2 mM DTT | 90% H2O/10% D2O | 50 | 6.5 | ambient | 298 | |