NMR Experiment |
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Experiment | Type | Sample Contents | Solvent | Ionic Strength | pH | Pressure | Temperature (K) | Spectrometer |
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1 | 2D 1H-15N HSQC | 0.9 mM [U-99% 13C; U-99% 15N] DrR147D-1, 10 % [U-2H] D2O-2, 90 % H2O-3, 50 uM DSS-4, 10 mM DTT-5, 200 mM sodium chloride-6, 0.02 % sodium azide-7, 5 mM calcium chloride-8, 20 mM ammonium acetate-9 | 90% H2O/10% D2O | 435 | 4.5 | ambient | 298 | |
2 | 2D 1H-13C HSQC | 0.9 mM [U-99% 13C; U-99% 15N] DrR147D-1, 10 % [U-2H] D2O-2, 90 % H2O-3, 50 uM DSS-4, 10 mM DTT-5, 200 mM sodium chloride-6, 0.02 % sodium azide-7, 5 mM calcium chloride-8, 20 mM ammonium acetate-9 | 90% H2O/10% D2O | 435 | 4.5 | ambient | 298 | |
3 | 3D HNCO | 0.9 mM [U-99% 13C; U-99% 15N] DrR147D-1, 10 % [U-2H] D2O-2, 90 % H2O-3, 50 uM DSS-4, 10 mM DTT-5, 200 mM sodium chloride-6, 0.02 % sodium azide-7, 5 mM calcium chloride-8, 20 mM ammonium acetate-9 | 90% H2O/10% D2O | 435 | 4.5 | ambient | 298 | |
4 | 3D HNCACB | 0.9 mM [U-99% 13C; U-99% 15N] DrR147D-1, 10 % [U-2H] D2O-2, 90 % H2O-3, 50 uM DSS-4, 10 mM DTT-5, 200 mM sodium chloride-6, 0.02 % sodium azide-7, 5 mM calcium chloride-8, 20 mM ammonium acetate-9 | 90% H2O/10% D2O | 435 | 4.5 | ambient | 298 | |
5 | 3D HNCA | 0.9 mM [U-99% 13C; U-99% 15N] DrR147D-1, 10 % [U-2H] D2O-2, 90 % H2O-3, 50 uM DSS-4, 10 mM DTT-5, 200 mM sodium chloride-6, 0.02 % sodium azide-7, 5 mM calcium chloride-8, 20 mM ammonium acetate-9 | 90% H2O/10% D2O | 435 | 4.5 | ambient | 298 | |
6 | 3D CBCA(CO)NH | 0.9 mM [U-99% 13C; U-99% 15N] DrR147D-1, 10 % [U-2H] D2O-2, 90 % H2O-3, 50 uM DSS-4, 10 mM DTT-5, 200 mM sodium chloride-6, 0.02 % sodium azide-7, 5 mM calcium chloride-8, 20 mM ammonium acetate-9 | 90% H2O/10% D2O | 435 | 4.5 | ambient | 298 | |
7 | 3D HBHA(CO)NH | 0.9 mM [U-99% 13C; U-99% 15N] DrR147D-1, 10 % [U-2H] D2O-2, 90 % H2O-3, 50 uM DSS-4, 10 mM DTT-5, 200 mM sodium chloride-6, 0.02 % sodium azide-7, 5 mM calcium chloride-8, 20 mM ammonium acetate-9 | 90% H2O/10% D2O | 435 | 4.5 | ambient | 298 | |
8 | 3D HCCH-COSY | 0.9 mM [U-99% 13C; U-99% 15N] DrR147D-1, 10 % [U-2H] D2O-2, 90 % H2O-3, 50 uM DSS-4, 10 mM DTT-5, 200 mM sodium chloride-6, 0.02 % sodium azide-7, 5 mM calcium chloride-8, 20 mM ammonium acetate-9 | 90% H2O/10% D2O | 435 | 4.5 | ambient | 298 | |
9 | 3D simultaneous NCaliCaro HH NOESY | 0.9 mM [U-99% 13C; U-99% 15N] DrR147D-1, 10 % [U-2H] D2O-2, 90 % H2O-3, 50 uM DSS-4, 10 mM DTT-5, 200 mM sodium chloride-6, 0.02 % sodium azide-7, 5 mM calcium chloride-8, 20 mM ammonium acetate-9 | 90% H2O/10% D2O | 435 | 4.5 | ambient | 298 | |
10 | 2D 1H-13C HSQC | 0.9 mM [U-5% 13C; U-99% 15N] DrR147D-10, 10 % [U-2H] D2O-11, 90 % H2O-12, 50 uM DSS-13, 10 mM DTT-14, 200 mM sodium chloride-15, 0.02 % sodium azide-16, 5 mM calcium chloride-17, 20 mM ammonium acetate-18 | 90% H2O/10% D2O | 435 | 4.5 | ambient | 298 | |