2K9D
Solution structure of the domain X of measle phosphoprotein
SOLUTION NMR
| NMR Experiment | ||||||||
|---|---|---|---|---|---|---|---|---|
| Experiment | Type | Sample Contents | Solvent | Ionic Strength | pH | Pressure | Temperature (K) | Spectrometer |
| 1 | 2D 1H-1H TOCSY | 0.0015 mM XD domain | 90% H2O/10% D2O | 7 | ambient | 300 | ||
| 2 | 2D 1H-1H NOESY | 0.0015 mM XD domain | 90% H2O/10% D2O | 7 | ambient | 300 | ||
| 3 | 3D 1H-15N NOESY | 0.00106 mM XD domain | 90% H2O/10% D2O | 7 | ambient | 300 | ||
| NMR Spectrometer Information | |||
|---|---|---|---|
| Spectrometer | Manufacturer | Model | Field Strength |
| 1 | Bruker | DRX | 500 |
| NMR Refinement | ||
|---|---|---|
| Method | Details | Software |
| torsion angle dynamics | ARIA | |
| NMR Ensemble Information | |
|---|---|
| Conformer Selection Criteria | structures with the lowest energy |
| Conformers Calculated Total Number | 40 |
| Conformers Submitted Total Number | 20 |
| Representative Model | 1 (closest to the average) |
| Computation: NMR Software | ||||
|---|---|---|---|---|
| # | Classification | Version | Software Name | Author |
| 1 | structure solution | ARIA | 1.2 | Linge, O'Donoghue and Nilges |
| 2 | refinement | ARIA | 1.2 | Linge, O'Donoghue and Nilges |
| 3 | chemical shift assignment | ARIA | 1.2 | Linge, O'Donoghue and Nilges |
