2JZW

How the HIV-1 nucleocapsid protein binds and destabilises the (-)primer binding site during reverse transcription

SOLUTION NMR

NMR Experiment
Experiment	Type	Sample Contents	Solvent	Ionic Strength	pH	Pressure	Temperature (K)	Spectrometer
1	2D 1H-1H NOESY	1 mM NCp7(12-55), 1 mM P(-)PBS, 3 mM ZnCl2, 30 mM sodium chloride, 0.2 mM MgCl2	90% H2O/10% D2O	30	6.5	ambient	293
2	2D 1H-1H TOCSY	1 mM NCp7(12-55), 1 mM P(-)PBS, 3 mM ZnCl2, 30 mM sodium chloride, 0.2 mM MgCl2	90% H2O/10% D2O	30	6.5	ambient	293
3	2D DQF-COSY	1 mM NCp7(12-55), 1 mM P(-)PBS, 3 mM ZnCl2, 30 mM sodium chloride, 0.2 mM MgCl2	90% H2O/10% D2O	30	6.5	ambient	293

NMR Spectrometer Information
Spectrometer	Manufacturer	Model	Field Strength
1	Bruker	AVANCE	600

NMR Refinement
Method	Details	Software
simulated annealing		XwinNMR

NMR Ensemble Information
Conformer Selection Criteria	structures with the least restraint violations,structures with the lowest energy
Conformers Calculated Total Number	100
Conformers Submitted Total Number	19
Representative Model	1 (closest to the average)

Computation: NMR Software
#	Classification	Version	Software Name	Author
1	collection	XwinNMR	3.0	Bruker Biospin
2	processing	XwinNMR	3.0	Bruker Biospin
3	refinement	Discover	2.98	Accelrys

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.