2JYS
Solution structure of Simian Foamy Virus (mac) protease
SOLUTION NMR
| NMR Experiment | ||||||||
|---|---|---|---|---|---|---|---|---|
| Experiment | Type | Sample Contents | Solvent | Ionic Strength | pH | Pressure | Temperature (K) | Spectrometer |
| 1 | 3D 1H-15N NOESY | 2mM [U-98% 13C; U-98% 15N] protein, 50mM sodium phosphate, 100mM sodium chloride, 0.5mM DTT | 90% H2O/10% D2O | 7.4 | ambient | 298 | ||
| 2 | 3D 1H-13C NOESY | 2mM [U-98% 13C; U-98% 15N] protein, 50mM sodium phosphate, 100mM sodium chloride, 0.5mM DTT | 90% H2O/10% D2O | 7.4 | ambient | 298 | ||
| NMR Spectrometer Information | |||
|---|---|---|---|
| Spectrometer | Manufacturer | Model | Field Strength |
| 1 | Bruker | AVANCE | 700 |
| NMR Refinement | ||
|---|---|---|
| Method | Details | Software |
| simulated annealing | X-PLOR-NIH | |
| NMR Ensemble Information | |
|---|---|
| Conformer Selection Criteria | structures with the lowest energy |
| Conformers Calculated Total Number | 120 |
| Conformers Submitted Total Number | 20 |
| Representative Model | 1 (lowest energy) |
| Computation: NMR Software | ||||
|---|---|---|---|---|
| # | Classification | Version | Software Name | Author |
| 1 | structure solution | X-PLOR-NIH | Schwieters, Kuszewski, Tjandra and Clore | |
| 2 | refinement | X-PLOR-NIH | Schwieters, Kuszewski, Tjandra and Clore | |
