2FL8
Fitting of the gp10 trimer structure into the cryoEM map of the bacteriophage T4 baseplate in the hexagonal conformation.
ELECTRON MICROSCOPY
| Sample |
|---|
| bacteriophage T4 baseplate |
| Specimen Preparation | |
|---|---|
| Sample Aggregation State | PARTICLE |
| Vitrification Instrument | |
| Cryogen Name | ETHANE |
| Sample Vitrification Details | |
| 3D Reconstruction | |
|---|---|
| Reconstruction Method | SINGLE PARTICLE |
| Number of Particles | 945 |
| Reported Resolution (Å) | 12 |
| Resolution Method | |
| Other Details | THE COORDINATES CONTAIN CA ONLY. RESIDUES 161-405 ARE MISSING. PLEASE SEE THE RELATED EMDB ENTRY FOR DETAILS OF THE EM RECONSTRUCTION. |
| Refinement Type | |
| Symmetry Type | POINT |
| Point Symmetry | C6 |
| Map-Model Fitting and Refinement | |||||
|---|---|---|---|---|---|
| Id | 1 (2FKK, 1S2E) | ||||
| Refinement Space | REAL | ||||
| Refinement Protocol | RIGID BODY FIT | ||||
| Refinement Target | correlation coefficient as defined by the program COLORES/SITUS | ||||
| Overall B Value | |||||
| Fitting Procedure | |||||
| Details | METHOD--laplacian filtered real space REFINEMENT PROTOCOL--rigid body | ||||
| Data Acquisition | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detector Type | KODAK SO-163 FILM | ||||||||
| Electron Dose (electrons/Å**2) | 25 | ||||||||
| Imaging Experiment | 1 |
|---|---|
| Date of Experiment | 2001-01-30 |
| Temperature (Kelvin) | |
| Microscope Model | FEI/PHILIPS CM300FEG/T |
| Minimum Defocus (nm) | 1200 |
| Maximum Defocus (nm) | 5000 |
| Minimum Tilt Angle (degrees) | |
| Maximum Tilt Angle (degrees) | |
| Nominal CS | |
| Imaging Mode | BRIGHT FIELD |
| Specimen Holder Model | |
| Nominal Magnification | 45000 |
| Calibrated Magnification | |
| Source | FIELD EMISSION GUN |
| Acceleration Voltage (kV) | 300 |
| Imaging Details |
| EM Software | ||
|---|---|---|
| Task | Software Package | Version |
| MODEL FITTING | SITUS COLORES | |
| RECONSTRUCTION | SPIDER | |
