2EWL
Solution structure of the C-terminal domain (monomer) of the HPV45 oncoprotein E7
SOLUTION NMR
NMR Experiment | ||||||||
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Experiment | Type | Sample Contents | Solvent | Ionic Strength | pH | Pressure | Temperature (K) | Spectrometer |
1 | 3D_15N-separated_NOESY | 1mM E7 U-15N, 20mM d-TRIS, 100mM NaCl, 90% H2O, 10% D2O | 90% H2O/10% D2O | 120mM | 7.4 | ambient | 298 | |
2 | 3D_13C-separated_NOESY | 1mM E7 U-15N, 13C, 20mM d-TRIS, 100mM NaCl, 90% H2O, 10% D2O | 90% H2O/10% D2O | 120mM | 7.4 | ambient | 298 | |
3 | HNHA | 1mM E7 U-15N, 20mM d-TRIS, 100mM NaCl, 90% H2O, 10% D2O | 90% H2O/10% D2O | 120mM | 7.4 | ambient | 298 |
NMR Spectrometer Information | |||
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Spectrometer | Manufacturer | Model | Field Strength |
1 | Varian | INOVA | 600 |
2 | Varian | INOVA | 750 |
NMR Refinement | ||
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Method | Details | Software |
distance geometry/simulated annealing by CYANA, energy minimisation by OPAL | VNMR |
NMR Ensemble Information | |
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Conformer Selection Criteria | target function |
Conformers Calculated Total Number | 100 |
Conformers Submitted Total Number | 15 |
Representative Model | 1 (closest to the average) |
Additional NMR Experimental Information | |
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Details | The structure was determined using heteronuclear triple-resonance NMR spectroscopy. |
Computation: NMR Software | ||||
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# | Classification | Version | Software Name | Author |
1 | processing | VNMR | 6.1 rev. C | |
2 | data analysis | XEASY | 1.3.9 | Xia and Bartels |
3 | structure solution | CYANA | 2.1 | Guntert et al |
4 | refinement | OPAL | 2.6 | Luginbuhl et al |