2C8I

Complex Of Echovirus Type 12 With Domains 1, 2, 3 and 4 Of Its Receptor Decay Accelerating Factor (Cd55) By Cryo Electron Microscopy At 16 A


ELECTRON MICROSCOPY
Sample
ECHOVIRUS TYPE 12 BOUND TO DECAY ACCELERATING FACTOR
Specimen Preparation
Sample Aggregation StatePARTICLE
Vitrification Instrument
Cryogen NameETHANE
Sample Vitrification DetailsSTAINED WITH AMMONIUM MOLYBDATE PH 7.2. VITRIFIED IN LIQUID ETHANE (CRYO-NEGATIVE STAIN)
Staining TypeNEGATIVE
Staining MaterialAmmonium Molybdate
Staining Details
3D Reconstruction
Reconstruction MethodSINGLE PARTICLE
Number of Particles1501
Reported Resolution (Å)14
Resolution Method
Other DetailsTHE SEQUENCE OF THE ECHOVIRUS CAPSID PROTEINS IS FROM EV11 BUT THE EM DENSITY INTO WHICH THE STRUCTURE WAS FITTED IS THAT OF EV12
Refinement Type
Symmetry TypePOINT
Point SymmetryI
Map-Model Fitting and Refinement
Id1 (1H8T)
Refinement SpaceREAL
Refinement ProtocolOTHER
Refinement TargetOPTIMAL CORRELATION
Overall B Value
Fitting Procedure
DetailsMETHOD--LOCAL CORRELATION REFINEMENT PROTOCOL--LOCAL CORRELATION
Data Acquisition
Detector TypeKODAK SO-163 FILM
Electron Dose (electrons/Å**2)
Imaging Experiment1
Date of Experiment2004-09-01
Temperature (Kelvin)100
Microscope ModelJEOL 1200
Minimum Defocus (nm)600
Maximum Defocus (nm)2800
Minimum Tilt Angle (degrees)
Maximum Tilt Angle (degrees)
Nominal CS3.4
Imaging ModeBRIGHT FIELD
Specimen Holder Model
Nominal Magnification30000
Calibrated Magnification29100
SourceLAB6
Acceleration Voltage (kV)120
Imaging Details
EM Software
TaskSoftware PackageVersion
RECONSTRUCTIONEM3DR2
RECONSTRUCTIONPFT2
Image Processing
CTF Correction TypeCTF Correction DetailsNumber of Particles SelectedParticle Selection Details
DEFOCUS PAIR IMAGES OF INDIVIDUAL PARTICLES