NMR Experiment |
---|
Experiment | Type | Sample Contents | Solvent | Ionic Strength | pH | Pressure | Temperature (K) | Spectrometer |
---|
1 | 3D_13C-separated_NOESY | 0.9 mM Ki67 FHA U-15N,13C; 1 mM hNIFK(226-269)3P unlabeled
5 mM HEPES, 5 mM DTT, 1 mM EDTA, 150 mM NaCl, pH 7.4 | 93% H2O/7% D2O | 150 mM NaCl | 7.4 | ambient | 293 | |
2 | 3D_15N-separated_NOESY | 0.9 mM Ki67 FHA U-15N,13C; 1 mM hNIFK(226-269)3P unlabeled
5 mM HEPES, 5 mM DTT, 1 mM EDTA, 150 mM NaCl, pH 7.4 | 93% H2O/7% D2O | 150 mM NaCl | 7.4 | ambient | 293 | |
3 | 3D_13C-separated_NOESY | 1 mM Ki67 FHA unlabled; 0.9 mM hNIFK(226-269)3P U-15N,13C
5 mM HEPES, 5 mM DTT, 1 mM EDTA, 150 mM NaCl, pH 7.4 | 93% H2O/7% D2O | 150 mM NaCl | 7.4 | ambient | 293 | |
4 | 3D_15N-separated_NOESY | 1 mM Ki67 FHA unlabled; 0.9 mM hNIFK(226-269)3P U-15N,13C
5 mM HEPES, 5 mM DTT, 1 mM EDTA, 150 mM NaCl, pH 7.4 | 93% H2O/7% D2O | 150 mM NaCl | 7.4 | ambient | 293 | |
5 | 3D_12C/14N-filtered_13C_separated NOESY | 0.9 mM Ki67 FHA U-15N,13C; 1 mM hNIFK(226-269)3P unlabeled
5 mM HEPES, 5 mM DTT, 1 mM EDTA, 150 mM NaCl, pH 7.4 | 93% H2O/7% D2O | 150 mM NaCl | 7.4 | ambient | 293 | |
6 | 3D_12C/14N-filtered_13C_separated NOESY | 1 mM Ki67 FHA unlabled; 0.9 mM hNIFK(226-269)3P U-15N,13C
5 mM HEPES, 5 mM DTT, 1 mM EDTA, 150 mM NaCl, pH 7.4 | 93% H2O/7% D2O | 150 mM NaCl | 7.4 | ambient | 293 | |