1UON

REOVIRUS POLYMERASE LAMBDA-3 LOCALIZED BY ELECTRON CRYOMICROSCOPY OF VIRIONS AT 7.6-A RESOLUTION

ELECTRON MICROSCOPY

Sample
REOVIRUS POLYMERASE LAMBDA-3

Specimen Preparation
Sample Aggregation State	PARTICLE

3D Reconstruction
Reconstruction Method	SINGLE PARTICLE
Number of Particles	7939
Reported Resolution (Å)	7.6
Resolution Method
Other Details	STRUCTURE OF REOVIRUS CORE (REINISCH ET AL. 2000 NATURE 404:960-967) (PDB ENTRY 1EJ6). PROTEIN ATOMS 9986, NUCLEIC ACID ATOMS 281, HETEROGEN ATOMS 86, ...	STRUCTURE OF REOVIRUS CORE (REINISCH ET AL. 2000 NATURE 404:960-967) (PDB ENTRY 1EJ6). PROTEIN ATOMS 9986, NUCLEIC ACID ATOMS 281, HETEROGEN ATOMS 86, SOLVENT ATOMS 350.
Refinement Type
Symmetry Type	POINT
Point Symmetry	I

Map-Model Fitting and Refinement
Id	1 (1UON)
Refinement Space
Refinement Protocol	RIGID BODY FIT
Refinement Target
Overall B Value
Fitting Procedure
Details	METHOD--SITUS (CHACON ET AL, 2002 J. MOL. BIOL. 317,375-394) WAS USED TO FIT THE X-RAY STRUCTURE OF REOVIRUS POLYMERASE (TAO ET AL. 2002 CELL 111, 73 ...	METHOD--SITUS (CHACON ET AL, 2002 J. MOL. BIOL. 317,375-394) WAS USED TO FIT THE X-RAY STRUCTURE OF REOVIRUS POLYMERASE (TAO ET AL. 2002 CELL 111, 733-745) (PDB CODE 1N35) INTO THE EM DENSITY OF THE 7.6-A RESOLUTION MAP OF REOVIRUS VIRIONS. THE COORDINATES OF THE FITTED REOVIRUS POLYMERASE WERE ALIGNED TO THE X-RAY STRUCTURE OF REOVIRUS CORE (REINISCH ET AL. 2000 NATURE 404,960-967) (PDB ENTRY 1EJ6). ALL MATRICES FOR BUILDING AN ICOSAHEDRON CAN BE FOUND IN 1EJ6.

Data Acquisition
Detector Type	KODAK SO-163 FILM	KODAK SO-163 FILM
Electron Dose (electrons/Å**2)	20

Imaging Experiment	1	2
Date of Experiment	1997-12-01	2002-04-01
Temperature (Kelvin)	97
Microscope Model	FEI/PHILIPS CM200T	FEI/PHILIPS CM300FEG/T
Minimum Defocus (nm)	1300
Maximum Defocus (nm)	3200
Minimum Tilt Angle (degrees)
Maximum Tilt Angle (degrees)
Nominal CS	2
Imaging Mode	BRIGHT FIELD	BRIGHT FIELD
Specimen Holder Model
Nominal Magnification	38000	45000
Calibrated Magnification	39200	47440
Source	FIELD EMISSION GUN	FIELD EMISSION GUN
Acceleration Voltage (kV)	200	300
Imaging Details

EM Software
Task	Software Package	Version
RECONSTRUCTION	PFT

Image Processing
CTF Correction Type	CTF Correction Details	Number of Particles Selected	Particle Selection Details
	INDIVIDUAL PARTICLES, PHASE AND AMPLITUDE

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.