1UDL
The solution structure of the fifth SH3 domain of intersectin 2 (KIAA1256)
SOLUTION NMR
| NMR Experiment | ||||||||
|---|---|---|---|---|---|---|---|---|
| Experiment | Type | Sample Contents | Solvent | Ionic Strength | pH | Pressure | Temperature (K) | Spectrometer |
| 1 | 3D_13C-separated_NOESY | 1.29mM SH3 domain, 20mM phosphate buffer, 100mM NaCl, 0.02% NaN3 | 90% H2O/10% D2O | 120mM | 6.0 | ambient | 298 | |
| 2 | 3D_15N-separated_NOESY | 1.29mM SH3 domain, 20mM phosphate buffer, 100mM NaCl, 0.02% NaN3 | 90% H2O/10% D2O | 120mM | 6.0 | ambient | 298 | |
| NMR Spectrometer Information | |||
|---|---|---|---|
| Spectrometer | Manufacturer | Model | Field Strength |
| 1 | Bruker | AVANCE | 800 |
| NMR Refinement | ||
|---|---|---|
| Method | Details | Software |
| torsion angle dynamics | NMRView | |
| NMR Ensemble Information | |
|---|---|
| Conformer Selection Criteria | target function |
| Conformers Calculated Total Number | 100 |
| Conformers Submitted Total Number | 20 |
| Representative Model | 1 (lowest target function) |
| Computation: NMR Software | ||||
|---|---|---|---|---|
| # | Classification | Version | Software Name | Author |
| 1 | data analysis | NMRView | 5.0.4 | BRUCE A. JOHNSON |
| 2 | processing | NMRPipe | 20020425 | FRANK DELAGLIO |
| 3 | refinement | CYANA | 2.0.17 | P.Guntert |
| 4 | collection | XwinNMR | 2.6 | BRUKER |
| 5 | data analysis | KUJIRA | 0.801 | NAOHIRO KOBAYASHI |
