1U6P
NMR Structure of the MLV encapsidation signal bound to the Nucleocapsid protein
SOLUTION NMR
| NMR Experiment | ||||||||
|---|---|---|---|---|---|---|---|---|
| Experiment | Type | Sample Contents | Solvent | Ionic Strength | pH | Pressure | Temperature (K) | Spectrometer |
| 1 | 2D NOESY | 1.2MM PSI MONOMERIC RNA GUA-15N,13C;10MM TRIS, 100% D2O | 10MM TRIS, 100% D2O | 10 mM Nacl | 7.0 | AMBIENT | 308 | |
| 2 | 2D TOCSY | 1.2MM PSI MONOMERIC RNA GUA-15N,13C;10MM TRIS, 100% D2O | 10MM TRIS, 100% D2O | 10 mM Nacl | 7.0 | AMBIENT | 308 | |
| 3 | 3D_13C-separated_NOESY | 1.2MM PSI MONOMERIC RNA GUA-15N,13C;10MM TRIS, 100% D2O | 10MM TRIS, 100% D2O | 10 mM Nacl | 7.0 | AMBIENT | 308 | |
| 4 | 3D_15N-separated_NOESY | 1.2MM PSI MONOMERIC RNA GUA-15N,13C;10MM TRIS, 100% D2O | 10MM TRIS, 100% D2O | 10 mM Nacl | 7.0 | AMBIENT | 308 | |
| 5 | 4D_13C/15N-separated_NOESY | 1.2MM PSI MONOMERIC RNA GUA-15N,13C;10MM TRIS, 100% D2O | 10MM TRIS, 100% D2O | 10 mM Nacl | 7.0 | AMBIENT | 308 | |
| 6 | 4D_13C-separated_NOESY | 1.2MM PSI MONOMERIC RNA GUA-15N,13C;10MM TRIS, 100% D2O | 10MM TRIS, 100% D2O | 10 mM Nacl | 7.0 | AMBIENT | 308 | |
| NMR Spectrometer Information | |||
|---|---|---|---|
| Spectrometer | Manufacturer | Model | Field Strength |
| 1 | Bruker | DRX | 800 |
| NMR Refinement | ||
|---|---|---|
| Method | Details | Software |
| TORSION ANGLE DYNAMICS, SIMULATED ANNEALING | XwinNMR | |
| NMR Ensemble Information | |
|---|---|
| Conformer Selection Criteria | structures with the least restraint violations |
| Conformers Calculated Total Number | |
| Conformers Submitted Total Number | 20 |
| Representative Model | 1 (lowest energy) |
| Computation: NMR Software | ||||
|---|---|---|---|---|
| # | Classification | Version | Software Name | Author |
| 1 | collection | XwinNMR | 2.6 | bruker |
| 2 | processing | NMRPipe | 2.1 | Delaglio F et.al |
| 3 | data analysis | NMRView | 5.0.3 | Johnson B |
| 4 | refinement | CYANA | Guntert | |
