1QPM
NMR STRUCTURE OF THE MU BACTERIOPHAGE REPRESSOR DNA-BINDING DOMAIN
SOLUTION NMR
| NMR Experiment | ||||||||
|---|---|---|---|---|---|---|---|---|
| Experiment | Type | Sample Contents | Solvent | Ionic Strength | pH | Pressure | Temperature (K) | Spectrometer |
| 1 | SEE PRIMARY REFERENCE | 1MM REPRESSOR U-15N,13C 50MM PHOSPHATE (PH 6.2); 100MM NACL; 2.5MM D-DTT; 93% H2O, 7% D2O | 100mM NACL | 6.2 | AMBIENT | 300.0 | ||
| NMR Spectrometer Information | |||
|---|---|---|---|
| Spectrometer | Manufacturer | Model | Field Strength |
| 1 | Bruker | DRX | 600 |
| 2 | Bruker | DRX | 500 |
| NMR Refinement | ||
|---|---|---|
| Method | Details | Software |
| DISTANCE GEOMETRY SIMULATED ANNEALING | INFORMATION IN PRIMARY REFERENCE | X-PLOR |
| NMR Ensemble Information | |
|---|---|
| Conformer Selection Criteria | STRUCTURES WITH ACCEPTABLE COVALENT GEOMETRY,STRUCTURES WITH FAVORABLE NON- BOND ENERGY,STRUCTURES WITH THE LEAST RESTRAINT VIOLATIONS,STRUCTURES WITH THE LOWEST ENERGY |
| Conformers Calculated Total Number | 50 |
| Conformers Submitted Total Number | 26 |
| Representative Model | 26 (minimized average structure) |
| Additional NMR Experimental Information | |
|---|---|
| Details | THE STRUCTURE WAS DETERMINED USING HETERONUCLEAR NMR METHODS. COMPLETE INFORMATION IN PRIMARY REFERENCE. |
| Computation: NMR Software | ||||
|---|---|---|---|---|
| # | Classification | Version | Software Name | Author |
| 1 | structure solution | X-PLOR | 3.843 | |
| 2 | refinement | X-PLOR | 3.843 | |
