1Q9F

NMR STRUCTURE OF THE OUTER MEMBRANE PROTEIN OMPX IN DHPC MICELLES


SOLUTION NMR
NMR Experiment
ExperimentTypeSample ContentsSolventIonic StrengthpHPressureTemperature (K)Spectrometer
13D_15N-SEPARATED_NOESY2MM OMPX U-15N,13C,2H; 2MM OMPX U-15N,13C,2H/L,V,ID1- 13CH3; 2MM OMPX U-15N,10%-13C20mM SODIUM PHOSPHATE, 100mM NACL6.5AMBIENT303
23D_13C-SEPARATED_NOESY2MM OMPX U-15N,13C,2H; 2MM OMPX U-15N,13C,2H/L,V,ID1- 13CH3; 2MM OMPX U-15N,10%-13C20mM SODIUM PHOSPHATE, 100mM NACL6.5AMBIENT303
33D_H(C)(CC)-TOCSY-(CO)-[15N,1H]-TROSY2MM OMPX U-15N,13C,2H; 2MM OMPX U-15N,13C,2H/L,V,ID1- 13CH3; 2MM OMPX U-15N,10%-13C20mM SODIUM PHOSPHATE, 100mM NACL6.5AMBIENT303
52D_[13C,1H]-HSQC2MM OMPX U-15N,13C,2H; 2MM OMPX U-15N,13C,2H/L,V,ID1- 13CH3; 2MM OMPX U-15N,10%-13C20mM SODIUM PHOSPHATE, 100mM NACL6.5AMBIENT303
NMR Spectrometer Information
SpectrometerManufacturerModelField Strength
1BrukerDRX800
2BrukerDRX750
3BrukerDRX500
NMR Refinement
MethodDetailsSoftware
TORSION ANGLE DYNAMICS (CANDID)AUTOMATIC IDENTIFICATION OF HYDROGEN BOND CONSTRAINTSOPALp
NMR Ensemble Information
Conformer Selection Criteriatarget function
Conformers Calculated Total Number400
Conformers Submitted Total Number20
Computation: NMR Software
#ClassificationVersionSoftware NameAuthor
1refinementOPALp2.6KORADI, R., LUGINBUHL, P.
2structure solutionCYANA1.0
3structure solutionTALOS