1HYW

SOLUTION STRUCTURE OF BACTERIOPHAGE LAMBDA GPW

SOLUTION NMR

NMR Experiment
Experiment	Type	Sample Contents	Solvent	Ionic Strength	pH	Pressure	Temperature (K)	Spectrometer
1	3D_13C-separated_NOESY	1.5mM gpW U-15N, 13C; 10mM phosphate buffer NA, 200mM NaCl,90% H2O,10% D2O	90% H2O/10% D2O	200mM NaCl	6.5	ambient	298
2	3D_15N-separated_NOESY	1.5mM gpW U-15N, 13C; 10mM phosphate buffer NA, 200mM NaCl,90% H2O,10% D2O	90% H2O/10% D2O	200mM NaCl	6.5	ambient	298
3	HNHA	1.5mM gpW U-15N, 13C; 10mM phosphate buffer NA, 200mM NaCl,90% H2O,10% D2O	90% H2O/10% D2O	200mM NaCl	6.5	ambient	298

NMR Spectrometer Information
Spectrometer	Manufacturer	Model	Field Strength
1	Varian	INOVA	500
2	Varian	INOVA	600

NMR Refinement
Method	Details	Software
distance geometry simulated annealing	The structures are based on a total of 1293 restraints, 1171 are NOE-derived distance constraints, 72 dihedral angle restraints,50 distance restraints from hydrogen bonds.	X-PLOR

NMR Ensemble Information
Conformer Selection Criteria	structures with the lowest energy
Conformers Calculated Total Number	50
Conformers Submitted Total Number	15

Additional NMR Experimental Information
Details	The structure was determined using triple-resonance NMR spectroscopy.

Computation: NMR Software
#	Classification	Version	Software Name	Author
1	structure solution	X-PLOR	3.851	Brunger
2	processing	NMRPipe	1.7	Delaglio
3	data analysis	NMRView	3.0	Johnson
4	refinement	X-PLOR	3.851	Brunger

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.