1H1J

The SAP domain is a DNA-Binding Domain Capable of Binding S/MAR DNA


SOLUTION NMR
NMR Experiment
ExperimentTypeSample ContentsSolventIonic StrengthpHPressureTemperature (K)Spectrometer
113C-FILTERED NOESY1.5MM150 mM6.81 atm298
2TOCSY1.5MM150 mM6.81 atm298
NMR Spectrometer Information
SpectrometerManufacturerModelField Strength
1BrukerDRX500
NMR Refinement
MethodDetailsSoftware
simulated annealingREFINEMENT DETAILS CAN BE FOUND IN THE JRNL CITATION ABOVE. RESIDUES 45-51 ARE DISORDERED WITH NO DEFINED STRUCTURE AND NOT INCLUDED IN THE COORDINATES.X-PLOR
NMR Ensemble Information
Conformer Selection CriteriaLOWEST NOE ENERGIES
Conformers Calculated Total Number50
Conformers Submitted Total Number20
Representative Model1 (n/a)
Additional NMR Experimental Information
DetailsSTRUCTURE DETERMINED USING STANDARD TRIPLE-RESONANCE AND 3D 1H,13C- NOESY/TOCSY EXPERIMENTS ON 13C, 15N-LABELED PROTEIN.
Computation: NMR Software
#ClassificationVersionSoftware NameAuthor
1refinementX-PLOR3.8BRUNGER
2structure solutionFelix
3structure solutionSparky
4structure solutionX-PLOR3.8