1GWP
STRUCTURE OF THE N-TERMINAL DOMAIN OF THE MATURE HIV-1 CAPSID PROTEIN
SOLUTION NMR
| NMR Experiment | ||||||||
|---|---|---|---|---|---|---|---|---|
| Experiment | Type | Sample Contents | Solvent | Ionic Strength | pH | Pressure | Temperature (K) | Spectrometer |
| 1 | HMQC-NOESY-HSQC | 10 mM | 5.5 | 1 atm | 298 | |||
| 2 | HSQC HNCA | 10 mM | 5.5 | 1 atm | 298 | |||
| 3 | HN(CO)CA | 10 mM | 5.5 | 1 atm | 298 | |||
| 4 | HNCO | 10 mM | 5.5 | 1 atm | 298 | |||
| 5 | HNHA HMQC-NOESY-HSQC | 10 mM | 5.5 | 1 atm | 298 | |||
| 6 | HSQC-NOESY-HSQC | 10 mM | 5.5 | 1 atm | 298 | |||
| 7 | HMQC-NOESY-HMQC | 10 mM | 5.5 | 1 atm | 298 | |||
| NMR Spectrometer Information | |||
|---|---|---|---|
| Spectrometer | Manufacturer | Model | Field Strength |
| 1 | Bruker | DMX | 600 |
| NMR Refinement | ||
|---|---|---|
| Method | Details | Software |
| torsion angle dynamics | TORSION ANGLE DYNAMICS FOLLOWED BY ENERGY MINIMIZATION. GUNTERT, P., MUMENTHALER, C. WUTHRICH, K. (1997). TORSION ANGLE DYNAMICS FOR NMR STRUCTURE CALCULATION WITH THE NEW PROGRAM DYANA. J. MOL. BIOL. 273, 283-298. | DYANA |
| NMR Ensemble Information | |
|---|---|
| Conformer Selection Criteria | LOWEST TARGET FUNCTION |
| Conformers Calculated Total Number | 100 |
| Conformers Submitted Total Number | 20 |
| Representative Model | 1 (n/a) |
| Additional NMR Experimental Information | |
|---|---|
| Details | HSQC HNCA, HN(CO)CA, HNCO, HNHA HMQC-NOESY-HSQC, HSQC-NOESY-HSQC, HMQC-NOESY-HMQC |
| Computation: NMR Software | ||||
|---|---|---|---|---|
| # | Classification | Version | Software Name | Author |
| 1 | refinement | DYANA | GUNTERT,WUTHRICH | |
| 2 | structure solution | DYANA | ||
