1EOQ

ROUS SARCOMA VIRUS CAPSID PROTEIN: C-TERMINAL DOMAIN

SOLUTION NMR

NMR Experiment
Experiment	Type	Sample Contents	Solvent	Ionic Strength	pH	Pressure	Temperature (K)	Spectrometer
1	3D_13C-separated_NOESY	U-15N,13C RSV CA(155-249) 50 mM Sodium phosphate pH 4.9 50 mM NaCl 1 mM EDTA 1 mM DTT	90% H20, 10%D20	50 mM NaCl	4.9	ambient	298
2	3D_15N-separated_NOESY	U-15N RSV CA(155-249) 50 mM Sodium phosphate pH 4.9 50 mM NaCl 1 mM EDTA 1 mM DTT	90% H20, 10%D20	50 mM NaCl	4.9	ambient	298
3	HNHA	U-15N RSV CA(155-249) 50 mM Sodium phosphate pH 4.9 50 mM NaCl 1 mM EDTA 1 mM DTT	90% H20, 10%D20	50 mM NaCl	4.9	ambient	298
4	HNHB	U-15N RSV CA(155-249) 50 mM Sodium phosphate pH 4.9 50 mM NaCl 1 mM EDTA 1 mM DTT	90% H20, 10%D20	50 mM NaCl	4.9	ambient	298
5	HNHA	U-15N RSV CA(155-249) (used for the 3D_15N-SEPARATED_NOESY, HNHA, HNHB expts) and U-15N/U-13C RSV CA(155-249) (used for the 3D_13C-SEPARATED_NOESY expt) both suspended in the same buffer (50 mM Sodium phosphate pH 4.9 50 mM NaCl 1 mM EDTA 1 mM DTT)	90% H20, 10%D20	50 mM NaCl	4.9	ambient	298

NMR Spectrometer Information
Spectrometer	Manufacturer	Model	Field Strength
1	Varian	UNITYPLUS	600

NMR Refinement
Method	Details	Software
torsion angle dynamics/simulated annealing		NMRPipe

NMR Ensemble Information
Conformer Selection Criteria
Conformers Calculated Total Number
Conformers Submitted Total Number	1

Additional NMR Experimental Information
Details	The structure was determined using triple-resonance NMR spectroscopy.

Computation: NMR Software
#	Classification	Version	Software Name	Author
1	processing	NMRPipe	1.6	Delaglio, F.
2	data analysis	ANSIG	3.3	Kraulis, P.
3	refinement	CNS	1.0	BRUNGER,ADAMS,CLORE,DELANO,GROS,GROSSE-KUNSTLEVE,JIANG,KUSZEWSKI,NILGES,PANNU,READ,RICE,SIMONSON,WARREN

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.