1E4P

Structure of the ribozyme substrate hairpin of Neurospora VS RNA. A close look at the cleavage site


SOLUTION NMR
NMR Experiment
ExperimentTypeSample ContentsSolventIonic StrengthpHPressureTemperature (K)Spectrometer
1NOESY 100 mM5.0302
2TOCSY 100 mM5.0302
3DQF-COSY 100 mM5.0302
41H-31P HETCOR 100 mM5.0302
51H-13C HMQC 100 mM5.0302
61H-31P HETERO-TOCSY-NOESY 100 mM5.0302
7NOESY-J 100 mM5.0302
NMR Spectrometer Information
SpectrometerManufacturerModelField Strength
1BrukerDRX500
2VarianINOVA600
3VarianINOVA750
NMR Refinement
MethodDetailsSoftware
TORSIAN ANGLE DYNAMICSX-PLOR
NMR Ensemble Information
Conformer Selection CriteriaLEAST RESTRAINT VIOLATION AND LOWEST ENERGY
Conformers Calculated Total Number100
Conformers Submitted Total Number20
Representative Model1 (n/a)
Computation: NMR Software
#ClassificationVersionSoftware NameAuthor
1refinementX-PLOR3.851BRUNGER
2structure solutionX-PLOR