1AFO
DIMERIC TRANSMEMBRANE DOMAIN OF HUMAN GLYCOPHORIN A, NMR, 20 STRUCTURES
SOLUTION NMR
| NMR Experiment | ||||||||
|---|---|---|---|---|---|---|---|---|
| Experiment | Type | Sample Contents | Solvent | Ionic Strength | pH | Pressure | Temperature (K) | Spectrometer |
| 1 | HNCA | 6.0 | 313 | |||||
| 2 | CBCACONH | 6.0 | 313 | |||||
| 3 | HCCH-TOCSY | 6.0 | 313 | |||||
| 4 | 15N NOESY-HSQC | 6.0 | 313 | |||||
| 5 | 13C NOESY-HSQC | 6.0 | 313 | |||||
| 6 | HNHA | 6.0 | 313 | |||||
| 7 | SPIN-ECHO DIFF CT HSQC | 6.0 | 313 | |||||
| NMR Spectrometer Information | |||
|---|---|---|---|
| Spectrometer | Manufacturer | Model | Field Strength |
| 1 | GE | OMEGA | 500 |
| 2 | Varian | VARIAN | 600 |
| NMR Refinement | ||
|---|---|---|
| Method | Details | Software |
| DISTANCE GEOMETRY SIMULATED ANNEALING HYBRID | X-PLOR | |
| NMR Ensemble Information | |
|---|---|
| Conformer Selection Criteria | LEAST RESTRAINT VIOLATIONS |
| Conformers Calculated Total Number | 38 |
| Conformers Submitted Total Number | 20 |
| Computation: NMR Software | ||||
|---|---|---|---|---|
| # | Classification | Version | Software Name | Author |
| 1 | refinement | X-PLOR | 3.1 | BRUNGER |
| 2 | structure calculation | X-PLOR | 3.1 | BRUNGER |
