Annotations: 6LPH

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
A	d6lpha_	Alpha and beta proteins (a+b)	Suppressor of Fused, N-terminal domain	Suppressor of Fused, N-terminal domain	automated matches	automated matches	Fruit fly (Drosophila melanogaster ) [TaxId: 7227 ],	SCOPe (2.08)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	PF05076	e6lphA1	A: a+b three layers	X: Suppressor of Fused, N-terminal domain (From Topology)	H: Suppressor of Fused, N-terminal domain (From Topology)	T: Suppressor of Fused, N-terminal domain	F: PF05076	ECOD (1.6)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A	PF05076	Suppressor of fused protein (SUFU) (SUFU)	Suppressor of fused protein (SUFU)	SUFU, encoding the human orthologue of Drosophila suppressor of fused, appears to have a conserved role in the repression of Hedgehog signaling. SUFU exerts its repressor role by physically interacting with GLI proteins in both the cytoplasm and the ...	SUFU, encoding the human orthologue of Drosophila suppressor of fused, appears to have a conserved role in the repression of Hedgehog signaling. SUFU exerts its repressor role by physically interacting with GLI proteins in both the cytoplasm and the nucleus [1]. SUFU has been found to be a tumour-suppressor gene that predisposes individuals to medulloblastoma by modulating the SHH signaling pathway [2]. Genomic contextual analysis of bacterial SUFU versions revealed that they are immunity proteins against diverse nuclease toxins in polymorphic toxin systems [3].	Domain
A	PF12470	Suppressor of Fused Gli/Ci N terminal binding domain (SUFU_C)	Suppressor of Fused Gli/Ci N terminal binding domain	-	Family

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A	Suppressor of fused homolog	transcription factor binding protein binding binding DNA-binding transcription factor binding molecular sequestering activity RNA polymerase II-specific DNA-binding transcription factor binding nuclear localization sequence binding signal sequence binding peptide binding	negative regulation of intracellular transport regulation of localization negative regulation of nucleocytoplasmic transport cellular localization regulation of biological process regulation of cellular process nucleocytoplasmic transport transport regulation of transport nuclear transport biological regulation negative regulation of biological process intracellular transport negative regulation of transport regulation of nucleocytoplasmic transport negative regulation of intracellular transport regulation of localization negative regulation of nucleocytoplasmic transport cellular localization regulation of biological process regulation of cellular process nucleocytoplasmic transport transport regulation of transport nuclear transport biological regulation negative regulation of biological process intracellular transport negative regulation of transport regulation of nucleocytoplasmic transport establishment of localization localization regulation of cellular localization establishment of localization in cell negative regulation of cellular process cellular process regulation of intracellular transport maintenance of protein location protein localization cellular macromolecule localization macromolecule localization cytoplasmic sequestering of protein maintenance of location cellular response to stimulus response to stimulus negative regulation of signaling signaling regulation of signal transduction negative regulation of cell communication cell communication negative regulation of smoothened signaling pathway signal transduction regulation of smoothened signaling pathway regulation of signaling cell surface receptor signaling pathway negative regulation of response to stimulus negative regulation of signal transduction smoothened signaling pathway regulation of response to stimulus regulation of cell communication	intracellular anatomical structure cytoplasm cellular anatomical entity cytosol protein-containing complex membrane-bounded organelle intracellular organelle organelle nucleus intracellular membrane-bounded organelle
B	Serine/threonine-protein kinase fused	binding adenyl ribonucleotide binding nucleotide binding purine ribonucleotide binding purine ribonucleoside triphosphate binding nucleoside phosphate binding ion binding purine nucleotide binding organic cyclic compound binding anion binding adenyl nucleotide binding ATP binding heterocyclic compound binding carbohydrate derivative binding binding adenyl ribonucleotide binding nucleotide binding purine ribonucleotide binding purine ribonucleoside triphosphate binding nucleoside phosphate binding ion binding purine nucleotide binding organic cyclic compound binding anion binding adenyl nucleotide binding ATP binding heterocyclic compound binding carbohydrate derivative binding ribonucleotide binding small molecule binding identical protein binding protein binding protein homodimerization activity protein dimerization activity protein serine/threonine kinase activity catalytic activity, acting on a protein kinase activity protein kinase activity transferase activity catalytic activity phosphotransferase activity, alcohol group as acceptor transferase activity, transferring phosphorus-containing groups protein serine kinase activity smoothened binding signaling receptor binding G protein-coupled receptor binding	cellular response to stimulus regulation of biological process regulation of cellular process cell surface receptor signaling pathway response to stimulus signaling biological regulation smoothened signaling pathway cell communication cellular process signal transduction cellular localization plasma membrane bounded cell projection organization cell projection organization cellular response to stimulus regulation of biological process regulation of cellular process cell surface receptor signaling pathway response to stimulus signaling biological regulation smoothened signaling pathway cell communication cellular process signal transduction cellular localization plasma membrane bounded cell projection organization cell projection organization transport intracellular transport establishment of localization protein-containing complex localization cellular component organization localization organelle organization establishment of localization in cell microtubule-based transport transport along microtubule cytoskeleton-dependent intracellular transport microtubule-based movement microtubule-based process cellular component organization or biogenesis cilium organization intraciliary transport developmental process multicellular organismal process blastoderm segmentation embryonic pattern specification segmentation pattern specification process embryo development regionalization multicellular organism development anatomical structure development segment polarity determination periodic partitioning regulation of localization nucleocytoplasmic transport positive regulation of nucleocytoplasmic transport regulation of transport nuclear transport positive regulation of biological process regulation of nucleocytoplasmic transport positive regulation of transport regulation of cellular localization positive regulation of intracellular transport positive regulation of cellular process regulation of intracellular transport animal organ development wing disc development imaginal disc pattern formation imaginal disc development wing disc pattern formation regulation of protein modification process positive regulation of protein metabolic process regulation of protein ubiquitination positive regulation of post-translational protein modification regulation of primary metabolic process positive regulation of metabolic process positive regulation of protein modification process organic substance metabolic process protein ubiquitination positive regulation of macromolecule metabolic process protein metabolic process regulation of protein modification by small protein conjugation or removal protein modification process positive regulation of protein modification by small protein conjugation or removal positive regulation of protein ubiquitination organonitrogen compound metabolic process regulation of post-translational protein modification regulation of protein metabolic process primary metabolic process regulation of macromolecule metabolic process macromolecule modification metabolic process macromolecule metabolic process post-translational protein modification regulation of metabolic process protein modification by small protein conjugation protein modification by small protein conjugation or removal sexual reproduction cellular developmental process reproductive process germarium-derived egg chamber formation oogenesis multicellular organismal reproductive process cell development cellular process involved in reproduction in multicellular organism developmental process involved in reproduction female gamete generation gamete generation anatomical structure morphogenesis cell differentiation anatomical structure formation involved in morphogenesis germ cell development cell surface receptor protein serine/threonine kinase signaling pathway cellular response to growth factor stimulus negative regulation of signaling regulation of transmembrane receptor protein serine/threonine kinase signaling pathway negative regulation of biological process regulation of signal transduction negative regulation of cellular response to growth factor stimulus negative regulation of BMP signaling pathway negative regulation of cell communication cellular response to endogenous stimulus negative regulation of cellular process BMP signaling pathway enzyme-linked receptor protein signaling pathway cellular response to BMP stimulus regulation of signaling regulation of BMP signaling pathway negative regulation of response to stimulus response to endogenous stimulus regulation of cellular response to growth factor stimulus negative regulation of signal transduction response to BMP regulation of response to stimulus regulation of cell communication transforming growth factor beta receptor superfamily signaling pathway response to growth factor negative regulation of transmembrane receptor protein serine/threonine kinase signaling pathway negative regulation of smoothened signaling pathway regulation of smoothened signaling pathway protein phosphorylation protein autophosphorylation phosphorylation cellular metabolic process phosphate-containing compound metabolic process phosphorus metabolic process	membrane-bounded organelle cell projection organelle cilium cellular anatomical entity plasma membrane bounded cell projection intracellular anatomical structure cytoplasm cytosol protein-containing complex Hedgehog signaling complex intracellular protein-containing complex

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A	IPR037181	Suppressor of fused, N-terminal	Homologous Superfamily
A	IPR007768	Suppressor of fused	Family
A	IPR038489	Suppressor of fused, C-terminal domain superfamily	Homologous Superfamily
A	IPR020941	Suppressor of fused-like domain	Domain
A	IPR016591	Suppressor of fused, eukaryotic	Family
A	IPR024314	Suppressor of fused C-terminal	Domain
B	IPR017441	Protein kinase, ATP binding site	Binding Site
B	IPR011009	Protein kinase-like domain superfamily	Homologous Superfamily
B	IPR000719	Protein kinase domain	Domain
B	IPR008271	Serine/threonine-protein kinase, active site	Active Site

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.